Journal ArticleDOI
Assay of proteins in the presence of interfering materials.
TLDR
The Lowry protein assay is a sensitive but highly nonspecific procedure that has been modified so that protein can be assayed in the presence of interfering chemicals.About:
This article is published in Analytical Biochemistry.The article was published on 1976-01-01. It has received 3135 citations till now. The article focuses on the topics: Lowry protein assay & Bicinchoninic acid assay.read more
Citations
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Protein haze formation in wines revisited. The stabilising effect of organic acids
Luís M. Batista,Sara Monteiro,Sara Monteiro,Virgílio Loureiro,Artur R. Teixeira,Ricardo B. Ferreira,Ricardo B. Ferreira +6 more
TL;DR: In this article, the effect on the wine protein haze potential of five organic acids commonly encountered in wines (L(+)tartaric, L()-malic, citric, succinic and gluconic acids) was assessed.
Journal ArticleDOI
Long-term evaluation of aluminum hydroxide-coated sand for removal of bacteria from wastewater
Jienan Chen,Steve Truesdail,Fuhua Lu,Guogen Zhan,Carter Belvin,Ben Koopman,Sam Farrah,Dinesh O. Shah +7 more
TL;DR: In this article, the effectiveness of sand coated with aluminum hydroxide by in situ precipitation was evaluated over a 4-month period during which the sand was exposed to wastewater, and the results suggest that, absent of biogrowth, the effective lifetime of the aluminum-hydroxide coated sand is approximately 4 months.
Journal ArticleDOI
Improving a Synechocystis-based photoautotrophic chassis through systematic genome mapping and validation of neutral sites
Filipe Pinto,Catarina C. Pacheco,Paulo J. Oliveira,Paulo J. Oliveira,Arnau Montagud,Andrew Landels,Narciso Couto,Phillip C. Wright,Javier F. Urchueguía,Paula Tamagnini +9 more
TL;DR: The genome mapping and validation of five neutral sites in the chromosome of Synechocystis sp.
Journal ArticleDOI
Experimental studies on the pulmonary surfactant. Reconstitution of surface-active material.
TL;DR: It is suggested that in the lipid-protein complex of SAM, two different proteins are present; a hydrophobic one in lipids and more hydrophilic ones in the water phase that have a close association with the former.
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Structural mapping of chloroplast coupling factor.
Brian Snyder,Gordon G. Hammes +1 more
TL;DR: Results indicate that all three nucleotide sites are approximately equal to 50 A from the light-labeled gamma-sulfhydryl, while the third site, which binds nucleotides reversibly under all conditions, is about 42-47 A apart.
References
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Journal Article
Protein Measurement with the Folin Phenol Reagent
TL;DR: Procedures are described for measuring protein in solution or after precipitation with acids or other agents, and for the determination of as little as 0.2 gamma of protein.
Journal ArticleDOI
Influence of sucrose on protein determination by the Lowry procedure
B. P. Gerhardt,Harry Beevers +1 more
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Interference by detergents, chelating agents, and buffers with the Lowry protein determination.
TL;DR: The effect of succinic acid, sodium citrate, and Bicine on the Lowry method changes depending on the concentration of the chemicals, but the changes were not as significant as in the case of the chemical mentioned above.
Journal ArticleDOI
Interferences by sulfhydryl, disulfide reagents and potassium ions on protein determination by Lowry's method.
TL;DR: The concentrations ofulfhydryl, disulfide reagents, and also potassium ions interfere with the protein determination by Lowry's method, but at moderate concentrations this interference can be overcome by running appropriate blanks.