Journal ArticleDOI
Assay of proteins in the presence of interfering materials.
TLDR
The Lowry protein assay is a sensitive but highly nonspecific procedure that has been modified so that protein can be assayed in the presence of interfering chemicals.About:
This article is published in Analytical Biochemistry.The article was published on 1976-01-01. It has received 3135 citations till now. The article focuses on the topics: Lowry protein assay & Bicinchoninic acid assay.read more
Citations
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Organometallic estrogens: synthesis, interaction with lamb uterine estrogen receptor, and detection by infrared spectroscopy.
TL;DR: The observation that the binding affinity of the steroid depends on which side of the steroidsal A ring the organometallic label is bound demonstrates the nonequivalence of the two sides of the A ring with respect to the receptor site.
Journal ArticleDOI
The 'strict' anaerobe Desulfovibrio gigas contains a membrane-bound oxygen-reducing respiratory chain.
Rita S. Lemos,Cláudio M. Gomes,Margarida Santana,Jean LeGall,Jean LeGall,António V. Xavier,Miguel Teixeira +6 more
TL;DR: This report shows that membranes from Desulfovibrio gigas grown in fumarate/sulfate contain a respiratory chain fully competent to reduce dioxygen to water, showing that the relationships between oxygen and anaerobes are much more complex than originally thought.
Journal ArticleDOI
Spectroscopic studies of Pyrococcus furiosus superoxide reductase: Implications for active-site structures and the catalytic mechanism
Michael D. Clay,Francis E. Jenney,Peter L. Hagedoorn,Graham N. George,Michael W. W. Adams,Michael K. Johnson +5 more
TL;DR: EPR and VTMCD studies provide evidence of azide, ferrocyanide, hydroxide, and cyanide binding via displacement of the glutamate ligand, and the polarizations deduced from MCD saturation magnetization studies indicate that the zero-field splitting axis corresponds to one of the axes with trans-histidyl ligands.
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A myotrophic protein from chick embryo extract: its purification, identity to transferrin, and indispensability for avian myogenesis.
Ichio,Ichiro Kimura,Eijiro Ozawa +2 more
TL;DR: Comparison of myotrophic activity of EE with that of Tf indicated the presence of other factors in EE which promote myogenic cell growth synergistically with Tf, and the relation of T f to nerve-derived myotroph proteins and other factorsIn EE is discussed.
Journal Article
Expression, induction, and catalytic activity of the ethanol-inducible cytochrome P450 (CYP2E1) in human fetal liver and hepatocytes.
TL;DR: The results indicate that CYP2E1 is present in human fetal liver, that the enzyme is functionally similar to CYP 2E1 from adults, and that fetal hepatocyte CYP11 is inducible in culture by xenobiotics, including ethanol.
References
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Journal Article
Protein Measurement with the Folin Phenol Reagent
TL;DR: Procedures are described for measuring protein in solution or after precipitation with acids or other agents, and for the determination of as little as 0.2 gamma of protein.
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Influence of sucrose on protein determination by the Lowry procedure
B. P. Gerhardt,Harry Beevers +1 more
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Interference by detergents, chelating agents, and buffers with the Lowry protein determination.
TL;DR: The effect of succinic acid, sodium citrate, and Bicine on the Lowry method changes depending on the concentration of the chemicals, but the changes were not as significant as in the case of the chemical mentioned above.
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Interferences by sulfhydryl, disulfide reagents and potassium ions on protein determination by Lowry's method.
TL;DR: The concentrations ofulfhydryl, disulfide reagents, and also potassium ions interfere with the protein determination by Lowry's method, but at moderate concentrations this interference can be overcome by running appropriate blanks.