Journal ArticleDOI
Assay of proteins in the presence of interfering materials.
TLDR
The Lowry protein assay is a sensitive but highly nonspecific procedure that has been modified so that protein can be assayed in the presence of interfering chemicals.About:
This article is published in Analytical Biochemistry.The article was published on 1976-01-01. It has received 3135 citations till now. The article focuses on the topics: Lowry protein assay & Bicinchoninic acid assay.read more
Citations
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Determination of the anomeric specificity of the Escherichia coli CTP:CMP-3-deoxy-D-manno-octulosonate cytidylyltransferase by 13C NMR spectroscopy.
TL;DR: The presence of peaks at 174.3 and 101.4 ppm indicates that KDO retains the beta-configuration when linked in CMP-KDO, and the magnitude of this coupling constant supports a diaxial relationship between these two groups.
Journal ArticleDOI
Insulin sensitizers improve learning and attenuate tau hyperphosphorylation and neuroinflammation in 3xTg-AD mice
TL;DR: The chronic treatment of 3xTg-AD mice with piog litazone and rosiglitazone improved spatial learning, enhanced AKT signaling, and attenuated tau hyperphosphorylation and neuroinflammation and shed new light on the possible mechanisms by which these two insulin sensitizers might be useful for treating AD.
Journal ArticleDOI
Myristic acid increases $\Delta$6-desaturase activity in cultured rat hepatocytes
Sophie Jan,Hervé Guillou,Sabine d'Andrea,Stéphanie Daval,Monique Bouriel,Vincent Rioux,Philippe Legrand +6 more
TL;DR: Data showed that cell treatment with 0.5 mmol·L -1 myristic acid during 43 h specifically increased ∆ 6-desaturase activity, reproducible for three substrates of ∆6- Desaturase, i.e. oleic acid, linoleic Acid and α-linoleic acid.
Journal ArticleDOI
Purification of brush border membrane by thiocyanate treatment.
TL;DR: Rat small intestinal brush border membranes are purified from brush borders by homogenization in relatively high concentrations of thiocyanate salts and are capable of secondary active sodium-dependent glucose transport as demonstrated by sodium gradient-supported overshooting glucose uptake.
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Complementary DNA cloning, sequencing and expression of an unusual dehydrin from blueberry floral buds
Amnon Levi,Ganesh R. Panta,Ganesh R. Panta,Cécile M. Parmentier,Mubarack M. Muthalif,Rajeev Arora,Savita Shanker,Lisa J. Rowland +7 more
TL;DR: Five high-confidence peptide sequences, ranging from 9 to 25 amino acids long, obtained from the 60-kDa dehydrin exactly matched sequences encoded within the cDNA clone, which indicated that it encodes a dehydrin with a native molecular mass of ∼ 40 kDa instead of 60 kDa.
References
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Journal Article
Protein Measurement with the Folin Phenol Reagent
TL;DR: Procedures are described for measuring protein in solution or after precipitation with acids or other agents, and for the determination of as little as 0.2 gamma of protein.
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Influence of sucrose on protein determination by the Lowry procedure
B. P. Gerhardt,Harry Beevers +1 more
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Interference by detergents, chelating agents, and buffers with the Lowry protein determination.
TL;DR: The effect of succinic acid, sodium citrate, and Bicine on the Lowry method changes depending on the concentration of the chemicals, but the changes were not as significant as in the case of the chemical mentioned above.
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Interferences by sulfhydryl, disulfide reagents and potassium ions on protein determination by Lowry's method.
TL;DR: The concentrations ofulfhydryl, disulfide reagents, and also potassium ions interfere with the protein determination by Lowry's method, but at moderate concentrations this interference can be overcome by running appropriate blanks.