Journal ArticleDOI
Assay of proteins in the presence of interfering materials.
Reads0
Chats0
TLDR
The Lowry protein assay is a sensitive but highly nonspecific procedure that has been modified so that protein can be assayed in the presence of interfering chemicals.About:
This article is published in Analytical Biochemistry.The article was published on 1976-01-01. It has received 3135 citations till now. The article focuses on the topics: Lowry protein assay & Bicinchoninic acid assay.read more
Citations
More filters
Journal ArticleDOI
Mitochondrial protein import: identification of processing peptidase and of PEP, a processing enhancing protein
Gerhard Hawlitschek,Helmut Schneider,Bernd Schmidt,Maximilian Tropschug,Franz-Ulrich Hartl,Walter Neupert +5 more
TL;DR: The authors' data indicate that MPP contains the catalytic site and that PEP has an enhancing function in mitochondria, and that the mitochondrial processing enzyme appears to represent a new type of "signal peptidase," different from the bacterial leader peptidases and the signal peptid enzyme of the endoplasmic reticulum.
Journal ArticleDOI
A coat subunit of Golgi-derived non-clathrin-coated vesicles with homology to the clathrin-coated vesicle coat protein beta-adaptin.
Tito Serafini,Gudrun Stenbeck,A. Brecht,F. Lottspeich,Lelio Orci,James E. Rothman,James E. Rothman,Felix T. Wieland +7 more
TL;DR: The identification of β-COP as the 110K protein explains the blocking of secretion by the drug brefeldin A, indicating that they may be structurally related.
Journal ArticleDOI
A Cytochrome f/b6 Complex of Five Polypeptides with Plastoquinol-Plastocyanin-Oxidoreductase Activity from Spinach Chloroplasts
Eduard C. Hurt,Günter Hauska +1 more
TL;DR: Characteristics of this activity with respect to substrate concentrations, pH, detergent effect and other parameters are described.
Journal ArticleDOI
A gamma-aminobutyric acid/benzodiazepine receptor complex of bovine cerebral cortex.
TL;DR: The gamma-aminobutyric acid/benzodiazepine receptor from bovine cerebral cortex was solubilized with sodium deoxycholate and purified by affinity chromatography on benzodiazepines-agarose and ion exchange chromatography and had a pharmacological profile that corresponds to the receptor specificity found in membranes and crude soluble extracts.
Journal ArticleDOI
Metabolic cross talk between cytosolic and plastidial pathways of isoprenoid biosynthesis: unidirectional transport of intermediates across the chloroplast envelope membrane
Julie Ann Bick,B. Markus Lange +1 more
TL;DR: It is reported that isolated chloroplasts, envelope membrane vesicles, and proteoliposomes prepared from the solubilized proteins of envelope membranes are capable of the efficient transport of isopentenyl diph phosphate and geranyl diphosphate, and the data suggest that plastid membranes possess a unidirectional proton symport system for the export of specific isoprenoid intermediates involved in the metabolic cross talk between cytosolic and pl
References
More filters
Journal Article
Protein Measurement with the Folin Phenol Reagent
TL;DR: Procedures are described for measuring protein in solution or after precipitation with acids or other agents, and for the determination of as little as 0.2 gamma of protein.
Journal ArticleDOI
Influence of sucrose on protein determination by the Lowry procedure
B. P. Gerhardt,Harry Beevers +1 more
Journal ArticleDOI
Interference by detergents, chelating agents, and buffers with the Lowry protein determination.
TL;DR: The effect of succinic acid, sodium citrate, and Bicine on the Lowry method changes depending on the concentration of the chemicals, but the changes were not as significant as in the case of the chemical mentioned above.
Journal ArticleDOI
Interferences by sulfhydryl, disulfide reagents and potassium ions on protein determination by Lowry's method.
TL;DR: The concentrations ofulfhydryl, disulfide reagents, and also potassium ions interfere with the protein determination by Lowry's method, but at moderate concentrations this interference can be overcome by running appropriate blanks.