Journal ArticleDOI
Assay of proteins in the presence of interfering materials.
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TLDR
The Lowry protein assay is a sensitive but highly nonspecific procedure that has been modified so that protein can be assayed in the presence of interfering chemicals.About:
This article is published in Analytical Biochemistry.The article was published on 1976-01-01. It has received 3135 citations till now. The article focuses on the topics: Lowry protein assay & Bicinchoninic acid assay.read more
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Energy metabolism of newly settled Ostrea edulis spat during metamorphosis
TL;DR: The results indicated that protein supplied most of the energy inO.
Journal ArticleDOI
Thymidylate synthetase purified to homogeneity from human leukemic cells.
TL;DR: Thymidylate synthetase (5,10-methylenetetrahydrofolate:dUMP C-methyltransferase, EC 2.1.45) from a human leukemic cell line has been purified to homogeneity with one-step affinity column chromatography and has a specific activity of 3.8 micron/min per mg of protein, which corresponds to a turnover number of 250.
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Therapeutic benefits of a component of coffee in a rat model of Alzheimer's disease
Gustavo Basurto-Islas,Julie Blanchard,Yunn Chyn Tung,José R. Fernández,Michael Voronkov,Maxwell Stock,Sherry Zhang,Jeffry B. Stock,Khalid Iqbal +8 more
TL;DR: It is raised the possibility that EHT may make a substantial contribution to the apparent neuroprotective benefits associated with coffee consumption as evidenced by numerous epidemiologic studies indicating that coffee drinkers have substantially lowered risk of developing AD.
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Purification and characterization of casein kinase II (CKII) from delta cka1 delta cka2 Saccharomyces cerevisiae rescued by Drosophila CKII subunits. The free catalytic subunit of casein kinase II is not toxic in vivo.
TL;DR: Overexpression of total casein kinase II activity from 6- to 18-fold is not toxic and indeed has no overt phenotypic consequences, even though production of large amounts of free catalytic subunit also appears to be without effect.
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Influence of EGTA on the apparent Ca2+ affinity of Mg2+-dependent, Ca2+-stimulated ATPase in the human erythrocyte membrane
TL;DR: Analysis of the kinetic data by a computerized nonlinear curve fitting procedure showed that a low Ca2+ affinity state of the enzyme was converted to a high Ca2- affinity state in the presence of EGTA, and the species associated with the enzyme interconversion appeared to be [CaEGTA]2-.
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Journal Article
Protein Measurement with the Folin Phenol Reagent
TL;DR: Procedures are described for measuring protein in solution or after precipitation with acids or other agents, and for the determination of as little as 0.2 gamma of protein.
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Influence of sucrose on protein determination by the Lowry procedure
B. P. Gerhardt,Harry Beevers +1 more
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Interference by detergents, chelating agents, and buffers with the Lowry protein determination.
TL;DR: The effect of succinic acid, sodium citrate, and Bicine on the Lowry method changes depending on the concentration of the chemicals, but the changes were not as significant as in the case of the chemical mentioned above.
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Interferences by sulfhydryl, disulfide reagents and potassium ions on protein determination by Lowry's method.
TL;DR: The concentrations ofulfhydryl, disulfide reagents, and also potassium ions interfere with the protein determination by Lowry's method, but at moderate concentrations this interference can be overcome by running appropriate blanks.