Journal ArticleDOI
Assay of proteins in the presence of interfering materials.
Reads0
Chats0
TLDR
The Lowry protein assay is a sensitive but highly nonspecific procedure that has been modified so that protein can be assayed in the presence of interfering chemicals.About:
This article is published in Analytical Biochemistry.The article was published on 1976-01-01. It has received 3135 citations till now. The article focuses on the topics: Lowry protein assay & Bicinchoninic acid assay.read more
Citations
More filters
Journal ArticleDOI
The abundance of calmodulin mRNAs is regulated in phosphorylase kinase-deficient skeletal muscle.
TL;DR: Comparisons of the abundance and heterogeneities in calmodulin mRNAs between normal and phosphorylase kinase-deficient skeletal muscles demonstrate that in normal tissue there are four species of cal modulin mRNA distinguished by their molecular weight, and in the deficient tissue none of them are preferentially reduced.
Journal ArticleDOI
Mitochondria contain a distinct DNA topoisomerase.
TL;DR: The enzyme has been shown to be distinctly mitochondrial, differentiated from the nuclear topoisomerase by its sensitivity to the intercalating drug, ethidium bromide, and to the non-intercalating trypanocidal drug, Berenil.
Journal ArticleDOI
Biochemical compounds’ dynamics during larval development of the carpet-shell clam Ruditapes decussatus (Linnaeus, 1758): effects of mono-specific diets and starvation
TL;DR: The present paper studies survival, growth and biochemical changes in the early developmental stages (from egg to pediveliger) of the clam Ruditapes decussatus in order to characterize the nutritional requirements and the transition from the endotrophic to the exotrophic phase.
Journal ArticleDOI
Shmt1 Heterozygosity Impairs Folate-Dependent Thymidylate Synthesis Capacity and Modifies Risk of Apcmin-Mediated Intestinal Cancer Risk
TL;DR: Cytoplasmic serine hydroxymethyltransferase (SHMT1) regulates the partitioning of folate-activated one-carbons between thymidylate and S-adenosylmethionine biosynthesis, which modifies susceptibility to intestinal cancer in Apc(min)(/+) mice.
Journal Article
Biochemical Characterization of Fluoropyrimidine-resistant Murine Leukemic Cell Lines
TL;DR: Twenty clones stably resistant to 5-fluorouridine-resistant cells have been isolated from L1210 or P388 murine leukemia cells by a one-step mutation and selection procedure to elucidate the mechanisms of resistance in these cells.
References
More filters
Journal Article
Protein Measurement with the Folin Phenol Reagent
TL;DR: Procedures are described for measuring protein in solution or after precipitation with acids or other agents, and for the determination of as little as 0.2 gamma of protein.
Journal ArticleDOI
Influence of sucrose on protein determination by the Lowry procedure
B. P. Gerhardt,Harry Beevers +1 more
Journal ArticleDOI
Interference by detergents, chelating agents, and buffers with the Lowry protein determination.
TL;DR: The effect of succinic acid, sodium citrate, and Bicine on the Lowry method changes depending on the concentration of the chemicals, but the changes were not as significant as in the case of the chemical mentioned above.
Journal ArticleDOI
Interferences by sulfhydryl, disulfide reagents and potassium ions on protein determination by Lowry's method.
TL;DR: The concentrations ofulfhydryl, disulfide reagents, and also potassium ions interfere with the protein determination by Lowry's method, but at moderate concentrations this interference can be overcome by running appropriate blanks.