Journal ArticleDOI
Assay of proteins in the presence of interfering materials.
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TLDR
The Lowry protein assay is a sensitive but highly nonspecific procedure that has been modified so that protein can be assayed in the presence of interfering chemicals.About:
This article is published in Analytical Biochemistry.The article was published on 1976-01-01. It has received 3135 citations till now. The article focuses on the topics: Lowry protein assay & Bicinchoninic acid assay.read more
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Protective effect of caffeic acid against beta-amyloid-induced neurotoxicity by the inhibition of calcium influx and tau phosphorylation.
TL;DR: It is suggested that caffeic acid protected the PC12 cells against Abeta-induced toxicity by attenuating the elevation of intracellular calcium levels and tau phosphorylation and the reduction of GSK-3beta activation.
Journal ArticleDOI
Extraction of protein from the macroalga Palmaria palmata
TL;DR: The application of food-grade polysaccharidase preparations may not be feasible for the extraction of intact P. palmata proteins because of the high enzyme:substrate required, according to the results presented herein.
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Identification of strictly conserved histidine and arginine residues as part of the active site in Petunia hybrida flavanone 3beta-hydroxylase.
Richard Lukačin,Lothar Britsch +1 more
TL;DR: Sequence alignment analysis of various 2-oxoglutarate-dependent dioxygenases and related enzymes revealed eight amino acid residues that seem to be strictly conserved within this group of enzymes, and concludes that His220, His278 and Asp222 constitute three of the possible ligands for iron binding in the active site of flavanone 3beta-hydroxylase.
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Role of glycosylation in hyperphosphorylation of tau in Alzheimer’s disease
TL;DR: It is found that the non‐hyperphosphorylated tau from AD brain but not normal brain tau was glycosylated, and in vitro phosphorylation indicated that the gly cosylated tAU was a better substrate for cAMP‐dependent protein kinase than the deglycosylation tau.
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Oxidation-reduction states of FMN and FAD in NADPH-cytochrome P-450 reductase during reduction by NADPH.
Daniel D. Oprian,Minor J. Coon +1 more
TL;DR: The results were shown to correspond to those predicted on the basis of a model for the rapid exchange of reducing equivalents between the two flavins, the distribution being governed at any time by the reduction potentials for the individual flavin half-reactions.
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Journal Article
Protein Measurement with the Folin Phenol Reagent
TL;DR: Procedures are described for measuring protein in solution or after precipitation with acids or other agents, and for the determination of as little as 0.2 gamma of protein.
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Influence of sucrose on protein determination by the Lowry procedure
B. P. Gerhardt,Harry Beevers +1 more
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Interference by detergents, chelating agents, and buffers with the Lowry protein determination.
TL;DR: The effect of succinic acid, sodium citrate, and Bicine on the Lowry method changes depending on the concentration of the chemicals, but the changes were not as significant as in the case of the chemical mentioned above.
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Interferences by sulfhydryl, disulfide reagents and potassium ions on protein determination by Lowry's method.
TL;DR: The concentrations ofulfhydryl, disulfide reagents, and also potassium ions interfere with the protein determination by Lowry's method, but at moderate concentrations this interference can be overcome by running appropriate blanks.