Journal ArticleDOI
Assay of proteins in the presence of interfering materials.
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TLDR
The Lowry protein assay is a sensitive but highly nonspecific procedure that has been modified so that protein can be assayed in the presence of interfering chemicals.About:
This article is published in Analytical Biochemistry.The article was published on 1976-01-01. It has received 3135 citations till now. The article focuses on the topics: Lowry protein assay & Bicinchoninic acid assay.read more
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Journal ArticleDOI
Different conformational states of the purified Ca2+-ATPase of the erythrocyte plasma membrane revealed by controlled trypsin proteolysis.
TL;DR: Linoleic acid, which stimulates the activity of the enzyme to the same levels obtained with calmodulin, greatly accelerates the rate of trypsin proteolysis, causing the virtual disappearance of all polypeptides in the 90-76-kDa region.
Book ChapterDOI
Isolation of P450 enzymes from human liver.
Judy L. Raucy,Jerome M. Lasker +1 more
TL;DR: This chapter discusses the isolation of P450 enzymes from human liver, and the genetically determined variations that exist in the structure and function of human P 450 enzymes are rarely encountered in experimental animals.
Journal ArticleDOI
Isolation and partial characterization of apolipoprotein (a) from human lipoprotein (a).
L J Seman,W C Breckenridge +1 more
TL;DR: It is concluded that apo (a) is distinct from other apolipoproteins owing to its low avidity for lipid and the nature of the interaction with apo B.
Journal ArticleDOI
Antioxidative responses of Ocimum basilicum to sodium chloride or sodium sulphate salinization
TL;DR: The higher sensitivity of basil to Na( 2)SO(4) was associated with an enhanced accumulation of H(2)O(2), an inhibition of APX, GR and POD activities and a lower regeneration of reduced ascorbate (AsA) and reduced glutathione (GSH).
Journal ArticleDOI
Purification and Characterization of 1-O-Acylceramide Synthase, a Novel Phospholipase A2 with Transacylase Activity
Akira Abe,James A. Shayman +1 more
TL;DR: Results strongly suggest that 1-O-acylceramide synthase is both a transacylase and a novel phospholipase A2.
References
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Journal Article
Protein Measurement with the Folin Phenol Reagent
TL;DR: Procedures are described for measuring protein in solution or after precipitation with acids or other agents, and for the determination of as little as 0.2 gamma of protein.
Journal ArticleDOI
Influence of sucrose on protein determination by the Lowry procedure
B. P. Gerhardt,Harry Beevers +1 more
Journal ArticleDOI
Interference by detergents, chelating agents, and buffers with the Lowry protein determination.
TL;DR: The effect of succinic acid, sodium citrate, and Bicine on the Lowry method changes depending on the concentration of the chemicals, but the changes were not as significant as in the case of the chemical mentioned above.
Journal ArticleDOI
Interferences by sulfhydryl, disulfide reagents and potassium ions on protein determination by Lowry's method.
TL;DR: The concentrations ofulfhydryl, disulfide reagents, and also potassium ions interfere with the protein determination by Lowry's method, but at moderate concentrations this interference can be overcome by running appropriate blanks.