Journal ArticleDOI
Assay of proteins in the presence of interfering materials.
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TLDR
The Lowry protein assay is a sensitive but highly nonspecific procedure that has been modified so that protein can be assayed in the presence of interfering chemicals.About:
This article is published in Analytical Biochemistry.The article was published on 1976-01-01. It has received 3135 citations till now. The article focuses on the topics: Lowry protein assay & Bicinchoninic acid assay.read more
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A novel phosphatidylcholine-hydrolyzing phospholipase C induced by phosphate starvation in Arabidopsis.
Yuki Nakamura,Koichiro Awai,Koichiro Awai,Tatsuru Masuda,Yasushi Yoshioka,Ken-ichiro Takamiya,Hiroyuki Ohta +6 more
TL;DR: The results suggest that NPC4 plays an important role in the supply of both inorganic phosphate and diacylglycerol from membrane-localized phospholipids that would be used for phosphate supplementation and the replacement of polar lipids in the root plasma membrane during phosphate deprivation.
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In situ localization of the hepatocytic Na+/Taurocholate cotransporting polypeptide in rat liver
TL;DR: Native Ntcp represents a glycoprotein of the basolateral hepatocyte plasma membrane with its C-terminal end facing the intracellular compartment, thus providing further proof for its bile acid transport function in rat liver.
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Accumulation of cyclin-dependent kinase 5 (cdk5) in neurons with early stages of Alzheimer's disease neurofibrillary degeneration
Jin-Jing Pei,Inge Grundke-Iqbal,Khalid Iqbal,Nenad Bogdanovic,Bengt Winblad,Richard F. Cowburn +5 more
TL;DR: Findings suggest that this kinase might be involved in the formation of NFTs at a relatively early stage in the neocortex.
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Antifolding activity of hsp60 couples protein import into the mitochondrial matrix with export to the intermembrane space.
Hans Koll,Bernard Guiard,Joachim Rassow,Joachim Ostermann,Arthur L. Horwich,Walter Neupert,Franz-Ulrich Hartl +6 more
TL;DR: Hsp60 combines two activities: catalysis of folding of proteins destined for the matrix, and maintaining proteins in an unfolded state to facilitate their channeling between the machineries for import and export across the inner membrane.
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Measuring plant protein with the Bradford assay : 1. Evaluation and standard method.
TL;DR: The suitability of the Bradford protein assay for measuring plant protein was evaluated and a standard method developed and techniques for increasing absorbance stability and changes in protein quality are reported.
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Journal Article
Protein Measurement with the Folin Phenol Reagent
TL;DR: Procedures are described for measuring protein in solution or after precipitation with acids or other agents, and for the determination of as little as 0.2 gamma of protein.
Journal ArticleDOI
Influence of sucrose on protein determination by the Lowry procedure
B. P. Gerhardt,Harry Beevers +1 more
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Interference by detergents, chelating agents, and buffers with the Lowry protein determination.
TL;DR: The effect of succinic acid, sodium citrate, and Bicine on the Lowry method changes depending on the concentration of the chemicals, but the changes were not as significant as in the case of the chemical mentioned above.
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Interferences by sulfhydryl, disulfide reagents and potassium ions on protein determination by Lowry's method.
TL;DR: The concentrations ofulfhydryl, disulfide reagents, and also potassium ions interfere with the protein determination by Lowry's method, but at moderate concentrations this interference can be overcome by running appropriate blanks.