Journal ArticleDOI
Assay of proteins in the presence of interfering materials.
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TLDR
The Lowry protein assay is a sensitive but highly nonspecific procedure that has been modified so that protein can be assayed in the presence of interfering chemicals.About:
This article is published in Analytical Biochemistry.The article was published on 1976-01-01. It has received 3135 citations till now. The article focuses on the topics: Lowry protein assay & Bicinchoninic acid assay.read more
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Monomers of the Neurospora plasma membrane H+-ATPase catalyze efficient proton translocation.
TL;DR: The results thus clearly demonstrate that 105,000-dalton monomers of the Neurospora plasma membrane H+-ATPase can catalyze efficient ATP hydrolysis-driven proton translocation.
Journal ArticleDOI
Derepression and partial insensitivity to carbon catabolite repression of the methanol dissimilating enzymes in Hansenula polymorpha.
L. Eggeling,H. Sahm +1 more
TL;DR: It was shown that the carbon catabolite insensitivity of the synthesis of methanol-specific enzymes is not limited to substrates that are slowly metabolized.
Journal ArticleDOI
Beta-amyloid disrupted synaptic vesicle endocytosis in cultured hippocampal neurons.
Brent L. Kelly,Adriana Ferreira +1 more
TL;DR: Abeta, the main component of senile plaques, caused ultrastructural changes indicative of impaired synaptic vesicle endocytosis in cultured hippocampal neurons that have been stimulated by depolarization with high potassium, and evidence was provided suggesting that Abeta-induced dynamin 1 depletion might play an important role in this process.
Journal ArticleDOI
Developmental Regulation of Protein O-GlcNAcylation, O-GlcNAc Transferase, and O-GlcNAcase in Mammalian Brain
Ying Liu,Xiaojing Li,Yang Yu,Jianhua Shi,Jianhua Shi,Zhihou Liang,Xiaoqin Run,Yi Li,Chun-ling Dai,Inge Grundke-Iqbal,Khalid Iqbal,Fei Liu,Fei Liu,Cheng-Xin Gong +13 more
TL;DR: A gradual decline of global protein O-GlcNAcylation during developmental stages and adulthood is found and correlated positively to the total protein phosphorylation at serine residues, but not at threonine residues.
Electrogenicity, pH-Dependence, andStoichiometry ofthe Proton-Sucrose Symport
TL;DR: In this article, the proton-sucrose symport was examined in plasmamembrane vesicles isolated from sugar beet leaves and the effect of membrane potential on ApH-dependent flux was observed.
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Journal Article
Protein Measurement with the Folin Phenol Reagent
TL;DR: Procedures are described for measuring protein in solution or after precipitation with acids or other agents, and for the determination of as little as 0.2 gamma of protein.
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Influence of sucrose on protein determination by the Lowry procedure
B. P. Gerhardt,Harry Beevers +1 more
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Interference by detergents, chelating agents, and buffers with the Lowry protein determination.
TL;DR: The effect of succinic acid, sodium citrate, and Bicine on the Lowry method changes depending on the concentration of the chemicals, but the changes were not as significant as in the case of the chemical mentioned above.
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Interferences by sulfhydryl, disulfide reagents and potassium ions on protein determination by Lowry's method.
TL;DR: The concentrations ofulfhydryl, disulfide reagents, and also potassium ions interfere with the protein determination by Lowry's method, but at moderate concentrations this interference can be overcome by running appropriate blanks.