Journal ArticleDOI
Assay of proteins in the presence of interfering materials.
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TLDR
The Lowry protein assay is a sensitive but highly nonspecific procedure that has been modified so that protein can be assayed in the presence of interfering chemicals.About:
This article is published in Analytical Biochemistry.The article was published on 1976-01-01. It has received 3135 citations till now. The article focuses on the topics: Lowry protein assay & Bicinchoninic acid assay.read more
Citations
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Microsomal cytochrome P-450 from neonatal pig testis. Purification and properties of A C21 steroid side-chain cleavage system (17 alpha-hydroxylase-C17,20 lyase).
Shizuo Nakajin,Peter F. Hall +1 more
TL;DR: The studies raise the possibility that one microsomal protein (cytochrome P-450) may possess two enzymatic activities (hydroxylase and lyase) that require NADPH and a flavoprotein P- 450 reductase.
Journal ArticleDOI
Assays for Determination of Protein Concentration
TL;DR: In this paper, the primary focus of this report is assay selection, emphasizing sample and buffer compatibility, and the fundamentals of generating protein assay standard curves and of data processing are considered, as are high-throughput adaptations of the more commonly used protein assays.
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Purification and characterization of human placental aromatase cytochrome P-450.
James T. Kellis,Larry E. Vickery +1 more
TL;DR: Aromatase cytochrome P-450, which catalyzes the conversion of androgens to estrogens, was purified from human placental microsomes and displays catalytic characteristics similar to the enzyme in intact microsome in the aromatization of androstenedione, 19-hydroxyandrosthenione and 19-oxoandrostenedion.
Journal ArticleDOI
Promotion of Hyperphosphorylation by Frontotemporal Dementia Tau Mutations
TL;DR: Findings taken together suggest that the mutations in tau might cause neurodegeneration by making the protein a more favorable substrate for hyperphosphorylation by brain protein kinases.
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Rat liver proteins capable of transferring phosphatidylethanolamine. Purification and transfer activity for other phospholipids and cholesterol.
B Bloj,D B Zilversmit +1 more
TL;DR: The fraction obtained after ammonium sulfate precipitation, gel filtration on Sephadex G-75, ion-exchange chromatography on CM-cellulose, ampholyte displacement chromatography, and heat treatment exhibited an 876-fold increase in its phosphatidylethanolamine transfer activity as compared with the postmitochondrial supernatant adjusted to pH 5.1.
References
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Journal Article
Protein Measurement with the Folin Phenol Reagent
TL;DR: Procedures are described for measuring protein in solution or after precipitation with acids or other agents, and for the determination of as little as 0.2 gamma of protein.
Journal ArticleDOI
Influence of sucrose on protein determination by the Lowry procedure
B. P. Gerhardt,Harry Beevers +1 more
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Interference by detergents, chelating agents, and buffers with the Lowry protein determination.
TL;DR: The effect of succinic acid, sodium citrate, and Bicine on the Lowry method changes depending on the concentration of the chemicals, but the changes were not as significant as in the case of the chemical mentioned above.
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Interferences by sulfhydryl, disulfide reagents and potassium ions on protein determination by Lowry's method.
TL;DR: The concentrations ofulfhydryl, disulfide reagents, and also potassium ions interfere with the protein determination by Lowry's method, but at moderate concentrations this interference can be overcome by running appropriate blanks.