Journal ArticleDOI
Assay of proteins in the presence of interfering materials.
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TLDR
The Lowry protein assay is a sensitive but highly nonspecific procedure that has been modified so that protein can be assayed in the presence of interfering chemicals.About:
This article is published in Analytical Biochemistry.The article was published on 1976-01-01. It has received 3135 citations till now. The article focuses on the topics: Lowry protein assay & Bicinchoninic acid assay.read more
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Loss of blast cell procoagulant activity and improvement of hemostatic variables in patients with acute promyelocytic leukemia administered all-trans-retinoic acid.
Anna Falanga,Licia Iacoviello,Virgilio Evangelista,D. Belotti,R. Consonni,Andria D'Orazio,L Robba,Maria Benedetta Donati,T Barbui +8 more
TL;DR: Assessment of the PCA of bone marrow blasts from APL patients on therapy with ATRA alone or associated with chemotherapy shows ATRA-induced remission is accompanied by a prompt improvement in the coagulationibleeding syndrome typical of this disease, thus influencing early hemorrhagic deaths.
Journal ArticleDOI
Adipose conversion of 3T3-L1 cells in a serum-free culture system depends on epidermal growth factor, insulin-like growth factor I, corticosterone, and cyclic AMP.
TL;DR: A culture system for 3T3-L1 preadipocytes based on a serum-free chemically defined medium containing fetuin, transferrin, and pantothenate and corticosterone and MIX induces maximal adipose conversion within 12-14 days, indicating that its effect is due to elevated cellular cAMP levels.
Journal ArticleDOI
The regulation of phosphorylation of τ in SY5Y neuroblastoma cells: the role of protein phosphatases
TL;DR: The data suggest that OA induced hyperphosphorylation of τ is probably the result of activated MAPK and cdks in addition to decreased PP‐2A and PP‐1 activities and that in SY5Y cells the OAinduced cell death is associated with a decrease in stable microtubules.
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Controlled proteolysis of the purified Ca2+-ATPase of the erythrocyte membrane. A correlation between the structure and the function of the enzyme.
TL;DR: The purified Ca2+-pumping ATPase of the erythrocyte plasma membrane has been subjected to a controlled proteolytic treatment with trypsin and a model is presented which postulates a major and a minor degradation pattern and rationalizes the pattern of proteolysis and of the [125I]ioazidocalmodulin binding observed.
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Journal Article
Protein Measurement with the Folin Phenol Reagent
TL;DR: Procedures are described for measuring protein in solution or after precipitation with acids or other agents, and for the determination of as little as 0.2 gamma of protein.
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Influence of sucrose on protein determination by the Lowry procedure
B. P. Gerhardt,Harry Beevers +1 more
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Interference by detergents, chelating agents, and buffers with the Lowry protein determination.
TL;DR: The effect of succinic acid, sodium citrate, and Bicine on the Lowry method changes depending on the concentration of the chemicals, but the changes were not as significant as in the case of the chemical mentioned above.
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Interferences by sulfhydryl, disulfide reagents and potassium ions on protein determination by Lowry's method.
TL;DR: The concentrations ofulfhydryl, disulfide reagents, and also potassium ions interfere with the protein determination by Lowry's method, but at moderate concentrations this interference can be overcome by running appropriate blanks.