Journal ArticleDOI
Assay of proteins in the presence of interfering materials.
Reads0
Chats0
TLDR
The Lowry protein assay is a sensitive but highly nonspecific procedure that has been modified so that protein can be assayed in the presence of interfering chemicals.About:
This article is published in Analytical Biochemistry.The article was published on 1976-01-01. It has received 3135 citations till now. The article focuses on the topics: Lowry protein assay & Bicinchoninic acid assay.read more
Citations
More filters
Journal ArticleDOI
Neuronal subsets express multiple high-molecular-weight cell-surface glycoconjugates defined by monoclonal antibodies Cat-301 and VC1.1
TL;DR: Immunoprecipitation experiments and biochemical characterizations indicate that Cat-301 and VC1.1 define at least 3 distinct high- molecular-weight antigens, and results indicate that structural microheterogeneity exists among Cat- 301 and VC 1.1 in the mammalian CNS.
Journal ArticleDOI
Regulation of the mitochondrial ATP synthase in intact rat cardiomyocytes.
A M Das,David A. Harris +1 more
TL;DR: It is proposed that mitochondria in vivo are directly regulated at the level of the ATP synthase, and that a regulator protein, the naturally occurring inhibitor protein from mitochondria, may be responsible for this regulation.
Journal ArticleDOI
Packaging Influenced Total Chlorophyll, Soluble Protein, Fatty Acid Composition and Lipoxygenase Activity in Broccoli Florets
TL;DR: In this article, modified atmosphere packaging (MAP), automatic misting (AM) and vent packaging (VP) were used to reduce postharvest deterioration of vegetables and fruits in broccoli.
Journal ArticleDOI
Photophysical properties, DNA photocleavage, and photocytotoxicity of a series of dppn dirhodium(II,II) complexes.
Lauren E. Joyce,J. Dafhne Aguirre,Alfredo M. Angeles-Boza,Abdellatif Chouai,Patty K.-L. Fu,Kim R. Dunbar,Claudia Turro +6 more
TL;DR: The ability of the complexes to bind and photocleave DNA was probed, along with their toxicity toward human skin cells in the dark and when irradiated with visible light, and their photophysical properties were investigated to probe their potential usefulness as photodynamic therapy agents.
Journal ArticleDOI
Elicitor induction of a microsomal 5-O-(4-coumaroyl)shikimate 3'-hydroxylase in parsley cell suspension cultures.
Werner Heller,Thomas Kühnl +1 more
TL;DR: Cytochrome c and carbon monoxide inhibit the hydroxylation reaction, suggesting a cytochrome P-450-dependent mixed-function monooxygenase.
References
More filters
Journal Article
Protein Measurement with the Folin Phenol Reagent
TL;DR: Procedures are described for measuring protein in solution or after precipitation with acids or other agents, and for the determination of as little as 0.2 gamma of protein.
Journal ArticleDOI
Influence of sucrose on protein determination by the Lowry procedure
B. P. Gerhardt,Harry Beevers +1 more
Journal ArticleDOI
Interference by detergents, chelating agents, and buffers with the Lowry protein determination.
TL;DR: The effect of succinic acid, sodium citrate, and Bicine on the Lowry method changes depending on the concentration of the chemicals, but the changes were not as significant as in the case of the chemical mentioned above.
Journal ArticleDOI
Interferences by sulfhydryl, disulfide reagents and potassium ions on protein determination by Lowry's method.
TL;DR: The concentrations ofulfhydryl, disulfide reagents, and also potassium ions interfere with the protein determination by Lowry's method, but at moderate concentrations this interference can be overcome by running appropriate blanks.