Journal ArticleDOI
Assay of proteins in the presence of interfering materials.
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TLDR
The Lowry protein assay is a sensitive but highly nonspecific procedure that has been modified so that protein can be assayed in the presence of interfering chemicals.About:
This article is published in Analytical Biochemistry.The article was published on 1976-01-01. It has received 3135 citations till now. The article focuses on the topics: Lowry protein assay & Bicinchoninic acid assay.read more
Citations
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Adenine nucleotide binding sites on beef heart F1-ATPase. Evidence for three exchangeable sites that are distinct from three noncatalytic sites.
R L Cross,C M Nalin +1 more
Journal ArticleDOI
COPI- and COPII-coated vesicles bud directly from the endoplasmic reticulum in yeast.
Sebastian Y. Bednarek,Mariella Ravazzola,Midori Hosobuchi,Mylène Amherdt,Alain Perrelet,Randy Schekman,Lelio Orci +6 more
TL;DR: The data suggest that COPI and COPII mediate separate vesicular transport pathways from the ER, which are devoid of endoplasmic reticulum (ER) resident proteins, and each contains targeting proteins necessary for docking at the Golgi complex.
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A filter paper dye-binding assay for quantitative determination of protein without interference from reducing agents or detergents.
TL;DR: This filter paper assay is useful for determining 100 ng to 20 micrograms of protein in the presence of ammonium sulfate, urea, thiol-reducing agents, amino acids, DNA, ionic and nonionic detergents, and acid or base.
Journal ArticleDOI
Galactolipid synthesis in chloroplast inner envelope is essential for proper thylakoid biogenesis, photosynthesis, and embryogenesis
TL;DR: The identification and characterization of an Arabidopsis mutant showing a complete defect in MGDG synthase 1 is reported, supporting an old hypothesis that envelope invagination is a major event in early chloroplast biogenesis and demonstrating the importance of galactolipids not only in photosynthetic growth but also in embryogenesis.
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Interaction of calcium and calmodulin in the presence of sodium dodecyl sulfate.
TL;DR: Calmodulin has been purified to homogeneity using an improved procedure that allows rapid processing of several kilograms of bovine brain and it was determined that calmodulin is not present as a soluble protein in Escherichia coli.
References
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Journal Article
Protein Measurement with the Folin Phenol Reagent
TL;DR: Procedures are described for measuring protein in solution or after precipitation with acids or other agents, and for the determination of as little as 0.2 gamma of protein.
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Influence of sucrose on protein determination by the Lowry procedure
B. P. Gerhardt,Harry Beevers +1 more
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Interference by detergents, chelating agents, and buffers with the Lowry protein determination.
TL;DR: The effect of succinic acid, sodium citrate, and Bicine on the Lowry method changes depending on the concentration of the chemicals, but the changes were not as significant as in the case of the chemical mentioned above.
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Interferences by sulfhydryl, disulfide reagents and potassium ions on protein determination by Lowry's method.
TL;DR: The concentrations ofulfhydryl, disulfide reagents, and also potassium ions interfere with the protein determination by Lowry's method, but at moderate concentrations this interference can be overcome by running appropriate blanks.