Journal ArticleDOI
Assay of proteins in the presence of interfering materials.
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TLDR
The Lowry protein assay is a sensitive but highly nonspecific procedure that has been modified so that protein can be assayed in the presence of interfering chemicals.About:
This article is published in Analytical Biochemistry.The article was published on 1976-01-01. It has received 3135 citations till now. The article focuses on the topics: Lowry protein assay & Bicinchoninic acid assay.read more
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Osmotin and thaumatin from grape: a putative general defense mechanism against pathogenic fungi.
Sara Monteiro,Mahmoud Barakat,Maria A. Piçarra-Pereira,Artur R. Teixeira,Ricardo B. Ferreira +4 more
TL;DR: Immunoblot analyses revealed the accumulation of the two PR proteins in infected leaves and berries, supporting a role in vivo in increasing the resistance of grapevine to fungal attack.
Journal ArticleDOI
Trypanosomatidae produce acetate via a mitochondrial acetate:succinate CoA transferase
TL;DR: A metabolic pathway common to mitochondria and hydrogenosomes is identified, which strongly supports a common origin for these two organelles.
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Adherence of Pseudomonas aeruginosa to human tracheobronchial mucin.
S Vishwanath,R Ramphal +1 more
TL;DR: It is inferred that there may be specific sites on human tracheobronchial mucin which facilitate this preferential binding of P. aeruginosa, and similar studies with strains of Escherichia coli and Klebsiella pneumoniae indicated a relative lack of binding of these bacteria to mucin.
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Influence of pH on Protein Interactions and Microstructure of Process Cheese
TL;DR: In this paper, a combination of microscopic and rheological techniques was used to understand the influence of pH on the stabilization of process cheese, and the contribution of ionic interactions to the stabilization was also studied.
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Cold responses of Arabidopsis mutants impaired in freezing tolerance
TL;DR: Mutants of Arabidopsis thaliana L. (Heynh), characterized as deficient in their freezing tolerance after cold acclimation, were surveyed for some of the normal responses to cold exposure, suggesting that they have other, perhaps highly specific, effects on low-temperature responses.
References
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Journal Article
Protein Measurement with the Folin Phenol Reagent
TL;DR: Procedures are described for measuring protein in solution or after precipitation with acids or other agents, and for the determination of as little as 0.2 gamma of protein.
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Influence of sucrose on protein determination by the Lowry procedure
B. P. Gerhardt,Harry Beevers +1 more
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Interference by detergents, chelating agents, and buffers with the Lowry protein determination.
TL;DR: The effect of succinic acid, sodium citrate, and Bicine on the Lowry method changes depending on the concentration of the chemicals, but the changes were not as significant as in the case of the chemical mentioned above.
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Interferences by sulfhydryl, disulfide reagents and potassium ions on protein determination by Lowry's method.
TL;DR: The concentrations ofulfhydryl, disulfide reagents, and also potassium ions interfere with the protein determination by Lowry's method, but at moderate concentrations this interference can be overcome by running appropriate blanks.