Journal ArticleDOI
Assay of proteins in the presence of interfering materials.
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TLDR
The Lowry protein assay is a sensitive but highly nonspecific procedure that has been modified so that protein can be assayed in the presence of interfering chemicals.About:
This article is published in Analytical Biochemistry.The article was published on 1976-01-01. It has received 3135 citations till now. The article focuses on the topics: Lowry protein assay & Bicinchoninic acid assay.read more
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An ATP-dependent Ca2+-pumping system in dog heart sarcolemma
P. Caroni,Ernesto Carafoli +1 more
TL;DR: In the present work, an ATP-dependent Ca2+ transport system which can be conclusively ascribed to heart sarcolemma is described and it is shown that a heart vesicular preparation particularly enriched in sar colemma markers accumulates Ca2- in the presence of ATP.
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Protein contents and physicochemical properties in honey samples of Apis mellifera of different floral origins
TL;DR: In this paper, the protein contents in honey samples of different floral origins, commercialized in several states of Brazil, were determined using the method of Bradford using the spectra of pollen of the honeys collected in those areas, in order to establish the correlation between the different botanical species and protein contents.
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The Generation of a 17 kDa Neurotoxic Fragment: An Alternative Mechanism by which Tau Mediates β-Amyloid-Induced Neurodegeneration
So-Young Park,Adriana Ferreira +1 more
TL;DR: The results suggest that Aβ-induced neurotoxicity may be mediated, at least in part, through the calpain-mediated generation of a toxic 17 kDa tau fragment, and provide insight into a novel mechanism by which tau could mediate A β- induced neurotoxicity.
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Dephosphorylation of Alzheimer Paired Helical Filaments by Protein Phosphatase-2A and −2B
TL;DR: It is demonstrated that PHF II-τ is accessible to dephosphorylation by PP-2A1 andPP-2B, and deph phosphorylation makes PHF dissociate, accessible to proteolysis by CANP, and biologically active in promoting the assembly of tubulin into microtubules.
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Isolation of an RNA-Directed RNA Polymerase–Specific cDNA Clone from Tomato
Winfried Schiebel,Thierry Pélissier,Leonhard Riedel,Sabine Thalmeir,Rosemarie Schiebel,Dirk Kempe,Friedrich Lottspeich,Heinz L. Sänger,Michael Wassenegger +8 more
TL;DR: Antibodies that were raised against synthetic peptides whose sequences have been deduced from the ORF were shown to specifically detect the 127-kD tomato RdRP protein.
References
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Journal Article
Protein Measurement with the Folin Phenol Reagent
TL;DR: Procedures are described for measuring protein in solution or after precipitation with acids or other agents, and for the determination of as little as 0.2 gamma of protein.
Journal ArticleDOI
Influence of sucrose on protein determination by the Lowry procedure
B. P. Gerhardt,Harry Beevers +1 more
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Interference by detergents, chelating agents, and buffers with the Lowry protein determination.
TL;DR: The effect of succinic acid, sodium citrate, and Bicine on the Lowry method changes depending on the concentration of the chemicals, but the changes were not as significant as in the case of the chemical mentioned above.
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Interferences by sulfhydryl, disulfide reagents and potassium ions on protein determination by Lowry's method.
TL;DR: The concentrations ofulfhydryl, disulfide reagents, and also potassium ions interfere with the protein determination by Lowry's method, but at moderate concentrations this interference can be overcome by running appropriate blanks.