Journal ArticleDOI
Assay of proteins in the presence of interfering materials.
TLDR
The Lowry protein assay is a sensitive but highly nonspecific procedure that has been modified so that protein can be assayed in the presence of interfering chemicals.About:
This article is published in Analytical Biochemistry.The article was published on 1976-01-01. It has received 3135 citations till now. The article focuses on the topics: Lowry protein assay & Bicinchoninic acid assay.read more
Citations
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Influence of polyunsaturated fats on composition of plasma lipoproteins and apolipoproteins.
TL;DR: The hypocholesterolemic effect of PUSF was uniform for all lipoproteins and usually was accompanied by a corresponding decrease in concentrations of apoprotein constituents and there were no changes in cholesterol/apoB or triglyceride-to-apoB ratios in VLDL despite a 23% decrease in plasma triglycerides on P USF.
Journal ArticleDOI
Secretome proteomics for discovery of cancer biomarkers.
TL;DR: This article focuses on the presentation of main methodological approaches that have been developed for the study of secreted proteins and the results thereof from the analysis of secretome in different types of malignancies; special emphasis is given on correlation of findings with protein expression in body fluids.
Journal ArticleDOI
A Non-transgenic Mouse Model (icv-STZ Mouse) of Alzheimer’s Disease: Similarities to and Differences from the Transgenic Model (3xTg-AD Mouse)
Yanxing Chen,Zhihou Liang,Julie Blanchard,Chun-ling Dai,Shenggang Sun,Moon Hee Lee,Inge Grundke-Iqbal,Khalid Iqbal,Fei Liu,Cheng-Xin Gong +9 more
TL;DR: Comparisons of the behavioral and neuropathological similarities and differences between the icv-STZ mouse and the 3xTg-AD mouse models will help guide future studies using these two mouse models for the development of AD drugs.
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Purification and characterization of an oxygen-insensitive NAD(P)H nitroreductase from Enterobacter cloacae.
TL;DR: Studies in which the rates of nitroreduction for a series of para substituted nitrobenzene derivatives were determined suggest that a linear free energy relationship exists between the rate and the redox midpoint potential of the substrate.
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A Factor X-Activating Cysteine Protease from Malignant Tissue
Stuart G. Gordon,Barbara Cross +1 more
TL;DR: Results suggest that cancer procoagulant is a cysteine endopeptidase, which distinguishes it from other coagulation factors including tissue factor, and suggest that neoplastic cells produce this unique Cysteine protease which may initiate blood coagulations.
References
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Journal Article
Protein Measurement with the Folin Phenol Reagent
TL;DR: Procedures are described for measuring protein in solution or after precipitation with acids or other agents, and for the determination of as little as 0.2 gamma of protein.
Journal ArticleDOI
Influence of sucrose on protein determination by the Lowry procedure
B. P. Gerhardt,Harry Beevers +1 more
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Interference by detergents, chelating agents, and buffers with the Lowry protein determination.
TL;DR: The effect of succinic acid, sodium citrate, and Bicine on the Lowry method changes depending on the concentration of the chemicals, but the changes were not as significant as in the case of the chemical mentioned above.
Journal ArticleDOI
Interferences by sulfhydryl, disulfide reagents and potassium ions on protein determination by Lowry's method.
TL;DR: The concentrations ofulfhydryl, disulfide reagents, and also potassium ions interfere with the protein determination by Lowry's method, but at moderate concentrations this interference can be overcome by running appropriate blanks.