Journal ArticleDOI
Assay of proteins in the presence of interfering materials.
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TLDR
The Lowry protein assay is a sensitive but highly nonspecific procedure that has been modified so that protein can be assayed in the presence of interfering chemicals.About:
This article is published in Analytical Biochemistry.The article was published on 1976-01-01. It has received 3135 citations till now. The article focuses on the topics: Lowry protein assay & Bicinchoninic acid assay.read more
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Characterization of the active site of a hydrogen sensor from Alcaligenes eutrophus
TL;DR: A third hydrogenase was recently identified in the proteobacterium Alcaligenes eutrophus as a constituent of a novel H2‐sensing multicomponent regulatory system and its catalytic power is low, but the RH is always active, insensitive to oxygen, and occurs in only two redox states.
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Disease modifying effect of chronic oral treatment with a neurotrophic peptidergic compound in a triple transgenic mouse model of Alzheimer's disease
Syed Faraz Kazim,Julie Blanchard,Chun-ling Dai,Yunn Chyn Tung,Frank M. LaFerla,Inge-Grundke Iqbal,Khalid Iqbal +6 more
TL;DR: The potential of the neurotrophic peptide mimetic as a disease modifying therapy for AD is demonstrated, with significant reduction in abnormal hyperphosphorylation and accumulation of tau at known major AD neurofibrillary pathology associated sites.
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The Lotus japonicus Sen1 gene controls rhizobial differentiation into nitrogen-fixing bacteroids in nodules
Norio Suganuma,Yasukazu Nakamura,M Yamamoto,Tazro Ohta,Hiroyuki Koiwa,Shoichiro Akao,Masayoshi Kawaguchi +6 more
TL;DR: Results indicate that Lj sym75 is required for bacterial differentiation into nitrogen-fixing bacteroids in nodules, and thus the Ljsym75 gene was renamed sen1 (for stationary endosymbiont nodule).
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A modular effector with a DNase domain and a marker for T6SS substrates.
TL;DR: A Vibrio antibacterial T6SS effector that contains a previously undescribed, widespread DNase toxin domain that is called PoNe (Polymorphic Nuclease effector), underline the modular nature of bacterial effectors harboring delivery or marker domains, specific to a secretion system, fused to interchangeable toxins.
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Hepatic HMGCoA reductase in human cholelithiasis: effects of chenodeoxycholic and ursodeoxycholic acids*
TL;DR: The activity of the rate‐limiting step in cholesterbgenesis, hydroxy‐methyl glutaryl co‐enzyme A reductase (HMGCoAR), was measured in operative wedge liver biopsies from ten patients with untreated cholesterol gallstones, six with pigment stones and ten controls.
References
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Journal Article
Protein Measurement with the Folin Phenol Reagent
TL;DR: Procedures are described for measuring protein in solution or after precipitation with acids or other agents, and for the determination of as little as 0.2 gamma of protein.
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Influence of sucrose on protein determination by the Lowry procedure
B. P. Gerhardt,Harry Beevers +1 more
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Interference by detergents, chelating agents, and buffers with the Lowry protein determination.
TL;DR: The effect of succinic acid, sodium citrate, and Bicine on the Lowry method changes depending on the concentration of the chemicals, but the changes were not as significant as in the case of the chemical mentioned above.
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Interferences by sulfhydryl, disulfide reagents and potassium ions on protein determination by Lowry's method.
TL;DR: The concentrations ofulfhydryl, disulfide reagents, and also potassium ions interfere with the protein determination by Lowry's method, but at moderate concentrations this interference can be overcome by running appropriate blanks.