Journal ArticleDOI
Assay of proteins in the presence of interfering materials.
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TLDR
The Lowry protein assay is a sensitive but highly nonspecific procedure that has been modified so that protein can be assayed in the presence of interfering chemicals.About:
This article is published in Analytical Biochemistry.The article was published on 1976-01-01. It has received 3135 citations till now. The article focuses on the topics: Lowry protein assay & Bicinchoninic acid assay.read more
Citations
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Expression of the human multidrug resistance cDNA in insect cells generates a high activity drug-stimulated membrane ATPase.
TL;DR: The expression of the human MDR1 gene in cultured Sf9 insect cells via a baculovirus vector generates a high activity vanadate-sensitive membrane ATPase, the first direct demonstration of a high capacity drug-stimulated ATPase activity of thehuman multidrug resistance protein.
Journal ArticleDOI
Taxol binds to polymerized tubulin in vitro
J. Parness,Susan Band Horwitz +1 more
TL;DR: It is suggested that taxol stabilizes interactions between dimers that lead to microtubule polymer formation and binds specifically and reversibly to a polymerized form of tubulin with a stoichiometry approaching unity.
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Antioxidative Defense System, Pigment Composition, and Photosynthetic Efficiency in Two Wheat Cultivars Subjected to Drought
TL;DR: Although differences in zeaxanthin content were not sufficient to explain the difference in drought tolerance between the two cultivars, zeaxAnthonyin formation may be relevant in avoiding irreversible damage to photosystem II in the more sensitive cultivar.
Journal ArticleDOI
Nitrogen fractions in selected feedstuffs
TL;DR: In this paper, Nitrogen in selected feedstuffs was fractionated based on solubility in mineral solvents and detergent solutions, and the results showed that Borate-phosphate buffer had a correlation coefficient of.92 with insoluble nitrogen obtained with autoclaved rumen fluid.
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St John's Wort induces intestinal P-glycoprotein/MDR1 and intestinal and hepatic CYP3A4
Donat Dürr,Bruno Stieger,Gerd A. Kullak-Ublick,Katharina Rentsch,Hans C. Steinert,Peter J. Meier,Karin Fattinger +6 more
TL;DR: Recently, St John's Wort was reported to substantially decrease blood/plasma concentrations and efficacy of cyclosporine (INN, ciclosporin), indinavir, and digoxin.
References
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Journal Article
Protein Measurement with the Folin Phenol Reagent
TL;DR: Procedures are described for measuring protein in solution or after precipitation with acids or other agents, and for the determination of as little as 0.2 gamma of protein.
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Influence of sucrose on protein determination by the Lowry procedure
B. P. Gerhardt,Harry Beevers +1 more
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Interference by detergents, chelating agents, and buffers with the Lowry protein determination.
TL;DR: The effect of succinic acid, sodium citrate, and Bicine on the Lowry method changes depending on the concentration of the chemicals, but the changes were not as significant as in the case of the chemical mentioned above.
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Interferences by sulfhydryl, disulfide reagents and potassium ions on protein determination by Lowry's method.
TL;DR: The concentrations ofulfhydryl, disulfide reagents, and also potassium ions interfere with the protein determination by Lowry's method, but at moderate concentrations this interference can be overcome by running appropriate blanks.