Journal ArticleDOI
Assay of proteins in the presence of interfering materials.
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TLDR
The Lowry protein assay is a sensitive but highly nonspecific procedure that has been modified so that protein can be assayed in the presence of interfering chemicals.About:
This article is published in Analytical Biochemistry.The article was published on 1976-01-01. It has received 3135 citations till now. The article focuses on the topics: Lowry protein assay & Bicinchoninic acid assay.read more
Citations
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Glucosidases involved in indole alkaloid biosynthesis of Catharanthus cell cultures
TL;DR: The discovery and characterization in C. roseus cell cultures of two highly glucoalkaloid-specific fi-glucosidases, which disprove the claims in [8].
Journal ArticleDOI
Regulation of follicle-stimulating hormone and dibutyryl adenosine 3',5'-monophosphate of a phosphodiesterase isoenzyme of the Sertoli cell.
TL;DR: FSH, after stimulation of intracellular cAMP, induces an increase in a high affinity phosphodiesterase and, therefore, an increased cAMP turnover in the Sertoli cell, which might be a relevant phenomenon in the control of the responsiveness of this cell to gonadotropin.
Journal ArticleDOI
Simultaneous expression of guinea pig UDP-glucuronosyltransferase 2B21 and 2B22 in COS-7 cells enhances UDP-glucuronosyltransferase 2B21-catalyzed morphine-6-glucuronide formation
Yuji Ishii,Aya Miyoshi,Ryoichi Watanabe,Kazuoki Tsuruda,M. Tsuda,Yuki Yamaguchi-Nagamatsu,Kunihiro Yoshisue,Mitsuko Tanaka,Daisuke Maji,Satoru Ohgiya,Kazuta Oguri +10 more
TL;DR: In this paper, a functional hetero-oligomer between UDP-glucuronosyltransferases (UGTs) and UGT2B21 cDNAs encoding guinea pig liver UGTs is provided by studies on the glucuronidation of morphine in transfected COS-7 cells.
Journal ArticleDOI
Development of a heavy metals enzymatic-based assay using papain
Yunus Shukor,Nor Azlan Baharom,Fadhil Abd. Rahman,Mohd Puad Abdullah,Nor Aripin Shamaan,Mohd Arif Syed +5 more
TL;DR: The papain assay is xenobiotics tolerant, has a wide pH for optimum activity, is temperature stable, and has a relatively quick assay time, and was used to identify polluted water samples from industrial sources in Penang, Malaysia.
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CTP:phosphorylcholine cytidylyltransferase from rat liver. Isolation and characterization of the catalytic subunit.
D A Feldman,P A Weinhold +1 more
TL;DR: The results suggested that a tetramer of the 45,000 protein possessed nearly optimal catalytic activity, and the need for stabilizing concentrations of Triton X-100 in the purified enzyme preparation may have prevented the association of the two proteins.
References
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Journal Article
Protein Measurement with the Folin Phenol Reagent
TL;DR: Procedures are described for measuring protein in solution or after precipitation with acids or other agents, and for the determination of as little as 0.2 gamma of protein.
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Influence of sucrose on protein determination by the Lowry procedure
B. P. Gerhardt,Harry Beevers +1 more
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Interference by detergents, chelating agents, and buffers with the Lowry protein determination.
TL;DR: The effect of succinic acid, sodium citrate, and Bicine on the Lowry method changes depending on the concentration of the chemicals, but the changes were not as significant as in the case of the chemical mentioned above.
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Interferences by sulfhydryl, disulfide reagents and potassium ions on protein determination by Lowry's method.
TL;DR: The concentrations ofulfhydryl, disulfide reagents, and also potassium ions interfere with the protein determination by Lowry's method, but at moderate concentrations this interference can be overcome by running appropriate blanks.