Journal ArticleDOI
Assay of proteins in the presence of interfering materials.
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TLDR
The Lowry protein assay is a sensitive but highly nonspecific procedure that has been modified so that protein can be assayed in the presence of interfering chemicals.About:
This article is published in Analytical Biochemistry.The article was published on 1976-01-01. It has received 3135 citations till now. The article focuses on the topics: Lowry protein assay & Bicinchoninic acid assay.read more
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Sequential processing of epidermal growth factor in early and late endosomes of rat liver.
C A Renfrew,Ann L. Hubbard +1 more
TL;DR: Results suggest that different proteases are active at selective points in the endocytic pathway and that the full complement of proteases needed for complete degradation of EGF is active only in lysosomes.
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Antibodies Against Denatured and Reduced Thyroid Microsomal Antigen in Autoimmune Thyroid Disease
TL;DR: It is demonstrated for the first clear demonstration that microsomal antibodies present in serum of patients with autoimmune thyroid disease are heterogeneous, and the data suggest that antibodies against denatured or Denatured and reduced MAg may be related to destruction of the thyroid gland.
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Human Liver Growth Hormone Receptor and Plasma Binding Protein: Characterization and Partial Purification*
TL;DR: The findings demonstrate the existence of multiple forms of the GH receptor in human liver (major components of 100 and 50-55 k, minor component of 130 k) and lend more support to the possible subunit structure of theGH receptor; they also suggest a close relationship, with common antigenic properties, of the membrane receptor and the plasma GH-BP.
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Two-dimensional electrophoretic analysis of Corynebacterium glutamicum membrane fraction and surface proteins.
Thomas Hermann,Melanie Finkemeier,Walter Pfefferle,Gregor Wersch,Reinhard Krämer,Andreas Burkovski +5 more
TL;DR: An improved protocol for the two‐dimensional analysis of proteins of the Corynebacterium glutamicum cytoplasmic membrane fraction was described and can now be separated into three distinct fractions resembling different functional units of the bacterial cell.
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Proteins as a potential nitrogen storage compound in bark and leaves of several softwoods
Suzanne Wetzel,John S. Greenwood +1 more
TL;DR: The total soluble protein content which was determined during active growth and during overwintering in the same tissues indicated that protein levels were higher in the winter compared to the summer in the bark of all species and in the leaves of Pinus spp.
References
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Journal Article
Protein Measurement with the Folin Phenol Reagent
TL;DR: Procedures are described for measuring protein in solution or after precipitation with acids or other agents, and for the determination of as little as 0.2 gamma of protein.
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Influence of sucrose on protein determination by the Lowry procedure
B. P. Gerhardt,Harry Beevers +1 more
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Interference by detergents, chelating agents, and buffers with the Lowry protein determination.
TL;DR: The effect of succinic acid, sodium citrate, and Bicine on the Lowry method changes depending on the concentration of the chemicals, but the changes were not as significant as in the case of the chemical mentioned above.
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Interferences by sulfhydryl, disulfide reagents and potassium ions on protein determination by Lowry's method.
TL;DR: The concentrations ofulfhydryl, disulfide reagents, and also potassium ions interfere with the protein determination by Lowry's method, but at moderate concentrations this interference can be overcome by running appropriate blanks.