Journal ArticleDOI
Assay of proteins in the presence of interfering materials.
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TLDR
The Lowry protein assay is a sensitive but highly nonspecific procedure that has been modified so that protein can be assayed in the presence of interfering chemicals.About:
This article is published in Analytical Biochemistry.The article was published on 1976-01-01. It has received 3135 citations till now. The article focuses on the topics: Lowry protein assay & Bicinchoninic acid assay.read more
Citations
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Fiber fermentability in the rat cecum and its physiological consequences
TL;DR: It appears that, besides analytical data on fiber solubility, the fermentability of complex fiber source is effectively appreciated by their effects on the parameters of cecal digestion.
Journal ArticleDOI
Direct electrochemistry of Megasphaera elsdenii iron hydrogenase. Definition of the enzyme's catalytic operating potential and quantitation of the catalytic behaviour over a continuous potential range.
TL;DR: The Fe-hydrogenase from Megasphaera elsdenii undergoes direct electron exchange with glassy carbon electrodes and Cyclic voltammetry defines the catalytic-performance of the enzyme over a continuous but precisely defined range of potentials.
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Metabolism of high density lipoprotein lipids by the rat liver: evidence for participation of hepatic lipase in the uptake of cholesteryl ester.
TL;DR: It is indicated that hepatic lipase is necessary for the hepatic uptake of both HDL triglycerides and cholesteryl esters and that the uptake of cholestery esters is not dependent on the hydrolysis of HDL triglyceride but is dependent onThe hydrolyzing of HDL phospholipids.
Journal ArticleDOI
An immunocytochemical and biochemical study of the microtubule-associated protein MAP-2 during post-lesion dendritic remodeling in the central nervous system of adult rats
TL;DR: Modifications in the levels and distribution of MAP-2 suggest that the dendritic cytoskeleton is dynamically changing in response to perturbation of the synaptic environment, and indicates that these changes may only occur in those neurons which have the capability to remodel their post-synaptic surface in Response to deafferentation.
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The formation of inositol phosphate derivatives by isolated membranes from Acer pseudoplatanus is stimulated by guanine nucleotides
M Dillenschneider,Alistair M. Hetherington,A. Graziana,Gilbert Alibert,Philippe Berta,Jacques Haiech,Raoul Ranjeva +6 more
TL;DR: It is suggested that GTP‐binding proteins exist in the Acer membrane and play a regulatory role in the formation of potentially important second messengers and were stimulatory in a dose‐dependent manner.
References
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Journal Article
Protein Measurement with the Folin Phenol Reagent
TL;DR: Procedures are described for measuring protein in solution or after precipitation with acids or other agents, and for the determination of as little as 0.2 gamma of protein.
Journal ArticleDOI
Influence of sucrose on protein determination by the Lowry procedure
B. P. Gerhardt,Harry Beevers +1 more
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Interference by detergents, chelating agents, and buffers with the Lowry protein determination.
TL;DR: The effect of succinic acid, sodium citrate, and Bicine on the Lowry method changes depending on the concentration of the chemicals, but the changes were not as significant as in the case of the chemical mentioned above.
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Interferences by sulfhydryl, disulfide reagents and potassium ions on protein determination by Lowry's method.
TL;DR: The concentrations ofulfhydryl, disulfide reagents, and also potassium ions interfere with the protein determination by Lowry's method, but at moderate concentrations this interference can be overcome by running appropriate blanks.