Journal ArticleDOI
Assay of proteins in the presence of interfering materials.
TLDR
The Lowry protein assay is a sensitive but highly nonspecific procedure that has been modified so that protein can be assayed in the presence of interfering chemicals.About:
This article is published in Analytical Biochemistry.The article was published on 1976-01-01. It has received 3135 citations till now. The article focuses on the topics: Lowry protein assay & Bicinchoninic acid assay.read more
Citations
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Intact growth factors are conserved in the extracellular matrix of ancient human bone and teeth: a storehouse for the study of human evolution in health and disease.
TL;DR: For the first time, growth factors, such as insulin growth factor II, bone morphogenetic protein-2 (BMP-2), and transforming growth factor-β (TGF-β), are extracted, solubilized and identified from archaeological compact human bone and tooth dentin dating from the late pre-ceramic pottery Neolithic and early Middle Ages.
Journal ArticleDOI
The addition of SDS to the Bradford dye-binding protein assay, a modification with increased sensitivity to collagen.
TL;DR: The addition of protein to a sub-threshold amount of SDS results in the formation of a green color measurable as an increase in absorbance at 700 nm, in contrast to the blue color measured at 595 nm in the standard assay.
Journal ArticleDOI
Quantitative Protein Precipitation from Guanidine Hydrochloride-Containing Solutions by Sodium Deoxycholate/Trichloroacetic Acid
TL;DR: The principles developed in the current study with ER will have broad applicability to splice variants of a diverse range of genes and be able to amplify the alternate sequences of the ER wild-type sequences.
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Antiapoptotic effect of ouabain on human umbilical vein endothelial cells.
TL;DR: The results show that ouabain has an antiapoptotic effect on HUVEC through the activation of PI-3K and ERK dependent pathways.
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Induction of neurite outgrowth in PC12 cells by the medium-chain fatty acid octanoic acid
TL;DR: The results indicate that MCFAs, including octanoic acid, induced neurite outgrowth of PC12 cells in the absence of NGF and suggest that the activation of p38 MAPK and ERK pathways is involved in this process.
References
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Journal Article
Protein Measurement with the Folin Phenol Reagent
TL;DR: Procedures are described for measuring protein in solution or after precipitation with acids or other agents, and for the determination of as little as 0.2 gamma of protein.
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Influence of sucrose on protein determination by the Lowry procedure
B. P. Gerhardt,Harry Beevers +1 more
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Interference by detergents, chelating agents, and buffers with the Lowry protein determination.
TL;DR: The effect of succinic acid, sodium citrate, and Bicine on the Lowry method changes depending on the concentration of the chemicals, but the changes were not as significant as in the case of the chemical mentioned above.
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Interferences by sulfhydryl, disulfide reagents and potassium ions on protein determination by Lowry's method.
TL;DR: The concentrations ofulfhydryl, disulfide reagents, and also potassium ions interfere with the protein determination by Lowry's method, but at moderate concentrations this interference can be overcome by running appropriate blanks.