Journal ArticleDOI
Assay of proteins in the presence of interfering materials.
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TLDR
The Lowry protein assay is a sensitive but highly nonspecific procedure that has been modified so that protein can be assayed in the presence of interfering chemicals.About:
This article is published in Analytical Biochemistry.The article was published on 1976-01-01. It has received 3135 citations till now. The article focuses on the topics: Lowry protein assay & Bicinchoninic acid assay.read more
Citations
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Isolation and characterization of a 30,000-dalton calcium-sensitive actin cross-linking protein from Dictyostelium discoideum.
Marcus Fechheimer,D L Taylor +1 more
TL;DR: Structural, immunological, and functional analyses demonstrated that the 30K protein was distinct from other actin-binding proteins of D. discoideum, and reversible cross-linking of actin filaments by the30K protein may contribute to regulation of the consistency and contractility of cytoplasm in D. Discoideum.
Journal ArticleDOI
Mthfd1 is an essential gene in mice and alters biomarkers of impaired one-carbon metabolism.
Amanda J. MacFarlane,Cheryll A. Perry,Hussein Girnary,Dacao Gao,Robert H. Allen,Sally P. Stabler,Barry Shane,Patrick J. Stover +7 more
TL;DR: The essentiality of the Mthfd1 gene is demonstrated and it is indicated that formate-derived 1Cs are utilized for de novo purine synthesis and the remethylation of homocysteine in liver, affirming the competition between thymidylate synthesis and homocy steine rem methylation for THF cofactors.
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An experimental rat model of sporadic Alzheimer's disease and rescue of cognitive impairment with a neurotrophic peptide
Silvia Bolognin,Julie Blanchard,Xiaochuan Wang,Gustavo Basurto-Islas,Yunn Chyn Tung,Erik Kohlbrenner,Inge Grundke-Iqbal,Khalid Iqbal +7 more
TL;DR: Peripheral administration of Peptide 6 rescued neurodegeneration and cognitive deficit in I2NTF–CTF animals by increasing dentate gyrus neurogenesis and mRNA level of brain derived neurotrophic factor.
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Detergent-solubilization, purification, and characterization of membrane-bound 3-hydroxy-3-methylglutaryl-coenzyme A reductase from radish seedlings
TL;DR: Study of enzyme stability and activation indicate that the enzyme is a hydrophobic protein with free thiol groups that are essential for full activity, and a sequential mechanism for the reduction of H MG-CoA to mevalonic acid with HMG- CoA being the first substrate binding to the enzyme, followed by NADPH.
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Isolation and characterization of a lipoprotein receptor from the fat body of an insect, Manduca sexta.
K Tsuchida,Michael A. Wells +1 more
TL;DR: It is proposed that the decrease in affinity of the receptor for lipoproteins depleted of diacylglycerol plays a key role in facilitating the transport of diACYl Glycerol from the midgut to the fat body during the larval feeding period.
References
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Journal Article
Protein Measurement with the Folin Phenol Reagent
TL;DR: Procedures are described for measuring protein in solution or after precipitation with acids or other agents, and for the determination of as little as 0.2 gamma of protein.
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Influence of sucrose on protein determination by the Lowry procedure
B. P. Gerhardt,Harry Beevers +1 more
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Interference by detergents, chelating agents, and buffers with the Lowry protein determination.
TL;DR: The effect of succinic acid, sodium citrate, and Bicine on the Lowry method changes depending on the concentration of the chemicals, but the changes were not as significant as in the case of the chemical mentioned above.
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Interferences by sulfhydryl, disulfide reagents and potassium ions on protein determination by Lowry's method.
TL;DR: The concentrations ofulfhydryl, disulfide reagents, and also potassium ions interfere with the protein determination by Lowry's method, but at moderate concentrations this interference can be overcome by running appropriate blanks.