Journal ArticleDOI
Assay of proteins in the presence of interfering materials.
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TLDR
The Lowry protein assay is a sensitive but highly nonspecific procedure that has been modified so that protein can be assayed in the presence of interfering chemicals.About:
This article is published in Analytical Biochemistry.The article was published on 1976-01-01. It has received 3135 citations till now. The article focuses on the topics: Lowry protein assay & Bicinchoninic acid assay.read more
Citations
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Journal ArticleDOI
The complexity of protein haze formation in wines
Luís M. Batista,Sara Monteiro,Sara Monteiro,Virgílio Loureiro,Artur R. Teixeira,Ricardo B. Ferreira,Ricardo B. Ferreira +6 more
TL;DR: In this paper, a series of heat stability tests were performed over a range of wine-relevant pH values (from 2.8 to 3.8), and the results indicated the existence of at least two different mechanisms responsible for the heat-induced precipitation of the Arinto wine proteins.
Journal ArticleDOI
Influence of Species of Vesicular-Arbuscular Mycorrhizal Fungi and Phosphorus Nutrition on Growth, Development, and Mineral Nutrition of Potato (Solanum tuberosum L.).
D. A. J. Mcarthur,N. R. Knowles +1 more
TL;DR: Plant P deficiency did not alter the shoot concentration of N, K, Mg, or Fe; however, the total accumulation of these mineral nutrients in shoots of P-stressed plants was substantially less than that of high-P plants.
Journal ArticleDOI
Measurement of total protein in plant samples in the presence of tannins
TL;DR: The Lowry method as modified by precipitation of proteins with trichloroacetic acid was found to be unsatisfactory for dried plant samples due to the incomplete extractability of proteins and interference with quantification of protein is likely to be minimal.
Journal ArticleDOI
Acyl carrier protein is present in the mitochondria of plants and eucaryotic micro‐organisms
Lorraine Chuman,Stuart Brody +1 more
TL;DR: Proteins antigenically similar to the acyl carrier protein (ACP) found in the mitochondria of Neurospora crassa were detected by immunoblotting and radioimmunoassay techniques in mitochondria isolated from yeast, potatoes, and pea leaves.
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The amino and carboxyl termini of the Neurospora plasma membrane H+-ATPase are cytoplasmically located.
S M Mandala,Carolyn W. Slayman +1 more
TL;DR: It is indicated that the ATPase polypeptide traverses the membrane an even number of times, in support of a previously published topological model.
References
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Journal Article
Protein Measurement with the Folin Phenol Reagent
TL;DR: Procedures are described for measuring protein in solution or after precipitation with acids or other agents, and for the determination of as little as 0.2 gamma of protein.
Journal ArticleDOI
Influence of sucrose on protein determination by the Lowry procedure
B. P. Gerhardt,Harry Beevers +1 more
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Interference by detergents, chelating agents, and buffers with the Lowry protein determination.
TL;DR: The effect of succinic acid, sodium citrate, and Bicine on the Lowry method changes depending on the concentration of the chemicals, but the changes were not as significant as in the case of the chemical mentioned above.
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Interferences by sulfhydryl, disulfide reagents and potassium ions on protein determination by Lowry's method.
TL;DR: The concentrations ofulfhydryl, disulfide reagents, and also potassium ions interfere with the protein determination by Lowry's method, but at moderate concentrations this interference can be overcome by running appropriate blanks.