Journal ArticleDOI
EGF-like growth factors as mediators of LH action in the ovulatory follicle.
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TLDR
It is demonstrated that LH stimulation induces the transient and sequential expression of the epidermal growth factor (EGF) family members amphiregulin, epire gulin, and beta-cellulin, which are paracrine mediators that propagate the LH signal throughout the follicle.Abstract:
Before ovulation in mammals, a cascade of events resembling an inflammatory and/or tissue remodeling process is triggered by luteinizing hormone (LH) in the ovarian follicle. Many LH effects, however, are thought to be indirect because of the restricted expression of its receptor. Here, we demonstrate that LH stimulation induces the transient and sequential expression of the epidermal growth factor (EGF) family members amphiregulin, epiregulin, and beta-cellulin. Incubation of follicles with these growth factors recapitulates the morphological and biochemical events triggered by LH, including cumulus expansion and oocyte maturation. Thus, these EGF-related growth factors are paracrine mediators that propagate the LH signal throughout the follicle.read more
Citations
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Journal ArticleDOI
MicroRNA-21 as a regulator of human cumulus cell viability and its potential influence on the developmental potential of the oocyte
TL;DR: Evidence is provided for a role of MicroRNA-21 in human cumulus cells that influences the developmental potential of human oocytes and the ability of the oocyte to fertilize and develop into a blastocyst, which is likely due in part to MIR-21-5p ability to promote cumulus cell viability by suppressing the expression of PTEN.
Journal ArticleDOI
Development of functional LH receptors on pig cumulus-oocyte complexes cultured in vitro by a novel two-step culture system.
TL;DR: The dbcAMP‐treated COCs represent a new model in which the resumption of meiosis and cumulus expansion can be induced exclusively by the action of recombinant LH.
Journal ArticleDOI
Regulation and action of early growth response 1 in bovine granulosa cells.
TL;DR: The regulation of E GR1 and EGR3 mRNA abundance by FGFs in granulosa cells is demonstrated and it is suggested that EGR1 is likely an upstream component of FGF signaling in granULosa cells.
Journal ArticleDOI
Epiregulin can effectively mature isolated cumulus-oocyte complexes, but fails as a substitute for the hCG/epidermal growth factor stimulus on cultured follicles.
Sergio Romero,Johan Smitz +1 more
TL;DR: Epiregulin has similar effects as EGF upon fully grown follicles and is as efficient as hCG/EGF, when used as meiotic stimulator for COCs isolated from the follicular environment.
Journal ArticleDOI
Intraovarian thrombin and activated protein C signaling system regulates steroidogenesis during the periovulatory period.
Yuan Cheng,Kazuhiro Kawamura,Kazuhiro Kawamura,Masashi Deguchi,Seido Takae,Sabine M. Mulders,Aaron J. W. Hsueh +6 more
TL;DR: Increased ovarian expression of key components of the thrombin-APC-PAR1/4 signaling system after LH/hCG stimulation is demonstrated, and this signaling pathway may allow optimal luteinization of preovulatory follicles.
References
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Journal ArticleDOI
Untangling the ErbB signalling network
Yosef Yarden,Mark X. Sliwkowski +1 more
TL;DR: When epidermal growth factor and its relatives bind the ErbB family of receptors, they trigger a rich network of signalling pathways, culminating in responses ranging from cell division to death, motility to adhesion.
PatentDOI
EGF receptor transactivation by G-protein-coupled receptors requires metalloproteinase cleavage of proHB-EGF
TL;DR: In this article, agents and methods for growth factor receptor activation by modulating the G-protein mediated signal transduction pathway were described, and a method to activate the growth factor receptors was proposed.
Journal ArticleDOI
Epidermal growth factor receptors: critical mediators of multiple receptor pathways.
TL;DR: The receptor for epidermal growth factor was identified as a downstream element in different signaling pathways, expanding its classical function as a receptor for EGF-like ligands to a role as mediator of diverse signaling systems and as a switch point of a cellular communication network.
Journal ArticleDOI
Targeted inactivation of the EGF and amphiregulin genes reveals distinct roles for EGF receptor ligands in mouse mammary gland development
Noreen C. Luetteke,Ting Hu Qiu,Suzanne E. Fenton,Kelly L. Troyer,Richard F. Riedel,Aileen Chang,David C. Lee +6 more
TL;DR: The results provide genetic confirmation of a requirement for EGFR signaling throughout the development of the mouse mammary gland, and reveal stage-dependent activities for different EGFR ligands.