Journal ArticleDOI
EGF-like growth factors as mediators of LH action in the ovulatory follicle.
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TLDR
It is demonstrated that LH stimulation induces the transient and sequential expression of the epidermal growth factor (EGF) family members amphiregulin, epire gulin, and beta-cellulin, which are paracrine mediators that propagate the LH signal throughout the follicle.Abstract:
Before ovulation in mammals, a cascade of events resembling an inflammatory and/or tissue remodeling process is triggered by luteinizing hormone (LH) in the ovarian follicle. Many LH effects, however, are thought to be indirect because of the restricted expression of its receptor. Here, we demonstrate that LH stimulation induces the transient and sequential expression of the epidermal growth factor (EGF) family members amphiregulin, epiregulin, and beta-cellulin. Incubation of follicles with these growth factors recapitulates the morphological and biochemical events triggered by LH, including cumulus expansion and oocyte maturation. Thus, these EGF-related growth factors are paracrine mediators that propagate the LH signal throughout the follicle.read more
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Journal ArticleDOI
Lutropin/choriogonadotropin stimulate the proliferation of primary cultures of rat Leydig cells through a pathway that involves activation of the extracellularly regulated kinase 1/2 cascade.
Koji Shiraishi,Mario Ascoli +1 more
TL;DR: It is concluded thatactivation of the LHR-provoked Leydig cell proliferation requires activation of the ERK1/2 cascade.
Journal ArticleDOI
Control of oocyte release by progesterone receptor-regulated gene expression
TL;DR: In ovarian granulosa cells, PGR may act as an inducible coregulator for constitutively bound Sp1/Sp3 transcription factors, which are key regulators for a discrete cohort of ovulatory genes.
Journal ArticleDOI
Sustained Activity of the EGF Receptor Is an Absolute Requisite for LH-Induced Oocyte Maturation and Cumulus Expansion
TL;DR: Light is shed on the unique characteristics of EGFR-ERK1/2 activity in the ovarian follicle and the fact that the ovulatory process involves a nonclassical activation of this pathway is emphasized.
Journal ArticleDOI
CNP/NPR2 signaling maintains oocyte meiotic arrest in early antral follicles and is suppressed by EGFR-mediated signaling in preovulatory follicles.
TL;DR: Examination of expression of Nppc and Npr2, respectively encoding CNP and NPR2, in the ovaries of immature mice suggests that CNP/NPR2 signaling is essential for oocyte meiotic arrest in early antral follicles and that activated LH/amphiregulin/EGFR signaling pathway suppresses this signal by downregulating NppC expression.
Journal ArticleDOI
Mutations in TUBB8 cause a multiplicity of phenotypes in human oocytes and early embryos
Ruizhi Feng,Zheng Yan,Bin Li,Min Yu,Qing Sang,Guoling Tian,Yao Xu,Biaobang Chen,Ronggui Qu,Zhaogui Sun,Xiaoxi Sun,Li Jin,Lin He,Yanping Kuang,Nicholas J. Cowan,Lei Wang +15 more
TL;DR: The data substantially expand the range of dysfunctional oocyte phenotypes incurred by mutation in TUBB8, underscore the independent nature of human oocyte meiosis and differentiation, extend the class of genetic diseases known as the tubulinopathies and provide new criteria for the qualitative evaluation of meiosis II (MII) oocytes for in vitro fertilization (IVF).
References
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Journal ArticleDOI
Untangling the ErbB signalling network
Yosef Yarden,Mark X. Sliwkowski +1 more
TL;DR: When epidermal growth factor and its relatives bind the ErbB family of receptors, they trigger a rich network of signalling pathways, culminating in responses ranging from cell division to death, motility to adhesion.
PatentDOI
EGF receptor transactivation by G-protein-coupled receptors requires metalloproteinase cleavage of proHB-EGF
TL;DR: In this article, agents and methods for growth factor receptor activation by modulating the G-protein mediated signal transduction pathway were described, and a method to activate the growth factor receptors was proposed.
Journal ArticleDOI
Epidermal growth factor receptors: critical mediators of multiple receptor pathways.
TL;DR: The receptor for epidermal growth factor was identified as a downstream element in different signaling pathways, expanding its classical function as a receptor for EGF-like ligands to a role as mediator of diverse signaling systems and as a switch point of a cellular communication network.
Journal ArticleDOI
Targeted inactivation of the EGF and amphiregulin genes reveals distinct roles for EGF receptor ligands in mouse mammary gland development
Noreen C. Luetteke,Ting Hu Qiu,Suzanne E. Fenton,Kelly L. Troyer,Richard F. Riedel,Aileen Chang,David C. Lee +6 more
TL;DR: The results provide genetic confirmation of a requirement for EGFR signaling throughout the development of the mouse mammary gland, and reveal stage-dependent activities for different EGFR ligands.