Journal ArticleDOI
EGF-like growth factors as mediators of LH action in the ovulatory follicle.
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TLDR
It is demonstrated that LH stimulation induces the transient and sequential expression of the epidermal growth factor (EGF) family members amphiregulin, epire gulin, and beta-cellulin, which are paracrine mediators that propagate the LH signal throughout the follicle.Abstract:
Before ovulation in mammals, a cascade of events resembling an inflammatory and/or tissue remodeling process is triggered by luteinizing hormone (LH) in the ovarian follicle. Many LH effects, however, are thought to be indirect because of the restricted expression of its receptor. Here, we demonstrate that LH stimulation induces the transient and sequential expression of the epidermal growth factor (EGF) family members amphiregulin, epiregulin, and beta-cellulin. Incubation of follicles with these growth factors recapitulates the morphological and biochemical events triggered by LH, including cumulus expansion and oocyte maturation. Thus, these EGF-related growth factors are paracrine mediators that propagate the LH signal throughout the follicle.read more
Citations
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Cyclic guanosine monophosphate does not inhibit gonadotropin-induced activation of mitogen-activated protein kinase 3/1 in pig cumulus-oocyte complexes
TL;DR: The findings of this study indicate that high cGMP concentrations inhibit the maturation of pig oocytes in vitro but the inhibitory mechanism does not involve the suppression of MAPK3/1 activation in cumulus cells.
Journal ArticleDOI
Meiotic Arrest In Vitro by Phosphodiesterase 3-Inhibitor Enhances Maturation Capacity of Human Oocytes and Allows Subsequent Embryonic Development
Daniela Nogueira,Raphael Ron-El,S. Friedler,M. Schachter,Arieh Raziel,Rita Cortvrindt,Johan Smitz +6 more
TL;DR: PMC with the specific PDE3-I had a beneficial effect on human CEOs by enhancing maturation, benefiting mainly the fully compacted CEOs, and this resulted in an increased yield of mature oocytes available for insemination without compromising embryonic development.
Journal ArticleDOI
The mare model for follicular maturation and reproductive aging in the woman.
TL;DR: The aim of this review is to evaluate the mare as an animal model to study age-associated changes in reproduction and to improve the understanding of oocyte and follicular maturation in vivo.
Journal ArticleDOI
Identification of new ovulation-related genes in humans by comparing the transcriptome of granulosa cells before and after ovulation triggering in the same controlled ovarian stimulation cycle
Marie Louise Muff Wissing,Stine Gry Kristensen,Claus Yding Andersen,Anne Lis Mikkelsen,T Høst,Rehannah Borup,Marie Louise Grøndahl +6 more
TL;DR: Genes related to inflammation, angiogenesis, extracellular matrix formation, growth factors and cancer were up-regulated while cell cycle genes were massively down-regulated, supporting the idea that the ovulation-related genes found in this study are relevant in the human natural cycle.
Journal ArticleDOI
Maternal heparin-binding-EGF deficiency limits pregnancy success in mice
Huirong Xie,Haibin Wang,Susanne Tranguch,Ryo Iwamoto,Eisuke Mekada,Francesco J. DeMayo,John P. Lydon,Sanjoy K. Das,Sudhansu K. Dey +8 more
TL;DR: It is shown here that maternal deficiency of heparin-binding EGF-like growth factor (HB-EGF) defers on-time implantation, leading to compromised pregnancy outcome, and it is demonstrated that amphiregulin, but not epireGulin, partially compensates for the loss of HB- EGF during implantation.
References
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Journal ArticleDOI
Untangling the ErbB signalling network
Yosef Yarden,Mark X. Sliwkowski +1 more
TL;DR: When epidermal growth factor and its relatives bind the ErbB family of receptors, they trigger a rich network of signalling pathways, culminating in responses ranging from cell division to death, motility to adhesion.
PatentDOI
EGF receptor transactivation by G-protein-coupled receptors requires metalloproteinase cleavage of proHB-EGF
TL;DR: In this article, agents and methods for growth factor receptor activation by modulating the G-protein mediated signal transduction pathway were described, and a method to activate the growth factor receptors was proposed.
Journal ArticleDOI
Epidermal growth factor receptors: critical mediators of multiple receptor pathways.
TL;DR: The receptor for epidermal growth factor was identified as a downstream element in different signaling pathways, expanding its classical function as a receptor for EGF-like ligands to a role as mediator of diverse signaling systems and as a switch point of a cellular communication network.
Journal ArticleDOI
Targeted inactivation of the EGF and amphiregulin genes reveals distinct roles for EGF receptor ligands in mouse mammary gland development
Noreen C. Luetteke,Ting Hu Qiu,Suzanne E. Fenton,Kelly L. Troyer,Richard F. Riedel,Aileen Chang,David C. Lee +6 more
TL;DR: The results provide genetic confirmation of a requirement for EGFR signaling throughout the development of the mouse mammary gland, and reveal stage-dependent activities for different EGFR ligands.