Journal ArticleDOI
EGF-like growth factors as mediators of LH action in the ovulatory follicle.
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TLDR
It is demonstrated that LH stimulation induces the transient and sequential expression of the epidermal growth factor (EGF) family members amphiregulin, epire gulin, and beta-cellulin, which are paracrine mediators that propagate the LH signal throughout the follicle.Abstract:
Before ovulation in mammals, a cascade of events resembling an inflammatory and/or tissue remodeling process is triggered by luteinizing hormone (LH) in the ovarian follicle. Many LH effects, however, are thought to be indirect because of the restricted expression of its receptor. Here, we demonstrate that LH stimulation induces the transient and sequential expression of the epidermal growth factor (EGF) family members amphiregulin, epiregulin, and beta-cellulin. Incubation of follicles with these growth factors recapitulates the morphological and biochemical events triggered by LH, including cumulus expansion and oocyte maturation. Thus, these EGF-related growth factors are paracrine mediators that propagate the LH signal throughout the follicle.read more
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Journal ArticleDOI
Epiregulin-dependent amphiregulin expression and ERBB2 signaling are involved in luteinizing hormone-induced paracrine signaling pathways in mouse ovary.
TL;DR: This study implicates that EREG mediates signals downstream of Areg mRNA expression and that EGFR-ERBB2 signals contributes to regulation of ovulation process.
Journal ArticleDOI
Establishment and validation of a model for non-luteinized human mural granulosa cell culture.
Libby Ophir,Yuval Yung,Yuval Yung,Ettie Maman,N. Rubinstein,Gil M. Yerushalmi,Jigal Haas,Eran Barzilay,Ariel Hourvitz +8 more
TL;DR: It is shown that early-non-luteinized, preovulatory- non-luteninized and luteal-MGCs have distinct gene expression pattern, which may provide a standardized research tool for delineation of the molecular processes occurring during the latter stages of follicular development in the human ovary.
Journal ArticleDOI
Neurotensin Enhances Sperm Capacitation and Acrosome Reaction in Mice
Yuuki Hiradate,Hiroki Inoue,Norio Kobayashi,Yoshiki Shirakata,Yutaka Suzuki,Aina Gotoh,Sang Gun Roh,Takafumi Uchida,Kazuo Katoh,Manabu Yoshida,Eimei Sato,Kentaro Tanemura +11 more
TL;DR: In this article, the expression and localization of NT receptors (NTR1) in mouse epididymal spermatozoa and investigated the effect of NT on sperm function.
Journal ArticleDOI
Nongenomic steroid-triggered oocyte maturation: of mice and frogs.
James Deng,Liliana Carbajal,Kristen Evaul,Melissa Rasar,Michelle Jamnongjit,Stephen R. Hammes +5 more
TL;DR: A brief summary of oocytes maturation is provided, focusing primarily on steroid-triggered meiotic progression in frogs and mice and new studies that implicate classical steroid receptors rather than alternative non-classical membrane steroid receptors as the primary regulators of steroid-mediated oocyte maturation in both of these model systems are presented.
Journal ArticleDOI
Influence of epidermal growth factor supplementation during in vitro maturation on nuclear status and gene expression of canine oocytes.
H.-J. Song,Eunju Kang,Geun-Ho Maeng,Sun-A Ock,S. L. Lee,Jae Gyu Yoo,Byeong-Gyun Jeon,Gyu-Jin Rho +7 more
TL;DR: The supplementation of 10 ng/ml EGF in IVM media exerted a positive influence on the progression of maturation to MII phase and the expression level of GJA5 at 72 h, but did not demonstrate any stimulatory role on the expression of EGFr and LHr during the maturation of the canine IVM oocytes.
References
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Journal ArticleDOI
Untangling the ErbB signalling network
Yosef Yarden,Mark X. Sliwkowski +1 more
TL;DR: When epidermal growth factor and its relatives bind the ErbB family of receptors, they trigger a rich network of signalling pathways, culminating in responses ranging from cell division to death, motility to adhesion.
PatentDOI
EGF receptor transactivation by G-protein-coupled receptors requires metalloproteinase cleavage of proHB-EGF
TL;DR: In this article, agents and methods for growth factor receptor activation by modulating the G-protein mediated signal transduction pathway were described, and a method to activate the growth factor receptors was proposed.
Journal ArticleDOI
Epidermal growth factor receptors: critical mediators of multiple receptor pathways.
TL;DR: The receptor for epidermal growth factor was identified as a downstream element in different signaling pathways, expanding its classical function as a receptor for EGF-like ligands to a role as mediator of diverse signaling systems and as a switch point of a cellular communication network.
Journal ArticleDOI
Targeted inactivation of the EGF and amphiregulin genes reveals distinct roles for EGF receptor ligands in mouse mammary gland development
Noreen C. Luetteke,Ting Hu Qiu,Suzanne E. Fenton,Kelly L. Troyer,Richard F. Riedel,Aileen Chang,David C. Lee +6 more
TL;DR: The results provide genetic confirmation of a requirement for EGFR signaling throughout the development of the mouse mammary gland, and reveal stage-dependent activities for different EGFR ligands.