Journal ArticleDOI
EGF-like growth factors as mediators of LH action in the ovulatory follicle.
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TLDR
It is demonstrated that LH stimulation induces the transient and sequential expression of the epidermal growth factor (EGF) family members amphiregulin, epire gulin, and beta-cellulin, which are paracrine mediators that propagate the LH signal throughout the follicle.Abstract:
Before ovulation in mammals, a cascade of events resembling an inflammatory and/or tissue remodeling process is triggered by luteinizing hormone (LH) in the ovarian follicle. Many LH effects, however, are thought to be indirect because of the restricted expression of its receptor. Here, we demonstrate that LH stimulation induces the transient and sequential expression of the epidermal growth factor (EGF) family members amphiregulin, epiregulin, and beta-cellulin. Incubation of follicles with these growth factors recapitulates the morphological and biochemical events triggered by LH, including cumulus expansion and oocyte maturation. Thus, these EGF-related growth factors are paracrine mediators that propagate the LH signal throughout the follicle.read more
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Journal ArticleDOI
Steady-state level of epidermal growth factor (EGF) mRNA and effect of EGF on in vitro culture of caprine preantral follicles
Juliana Jales de Hollanda Celestino,Jamily Bezerra Bruno,Márcia Viviane Alves Saraiva,R.M.P. Rocha,I.R. Brito,Ana Beatriz Graça Duarte,Valdevane Rocha Araújo,Cleidson Manoel Gomes da Silva,Maria Helena Tavares de Matos,Cláudio Cabral Campello,José Vieira Silva,José Ricardo de Figueiredo +11 more
TL;DR: EGF mRNA levels are higher in secondary follicles than in earlier stages, with both FSH and EGF promoting the growth of goat primary follicles and both EGF and FSH reduced FSH-R mRNA levels after follicle culture for 6 days.
Journal ArticleDOI
Transactivation of the epidermal growth factor receptor is involved in the lutropin receptor-mediated down-regulation of ovarian aromatase expression in vivo.
TL;DR: In vivo evidence is provided that down-regulation of Cyp19a1 by the LH surge requires efficient signaling through the epidermal growth factor receptor (EGFR), the first in vivo evidence linking the LH receptor, the EGFR, and ERK1/2 as sequential components of a pathway that regulates ovarian Cyp 19a1 expression.
Journal ArticleDOI
Differences in Collagen Expression in Cumulus Cells after Exposure to Highly Purified Menotropin or Recombinant Follicle-Stimulating Hormone in a Mouse Follicle Culture Model
TL;DR: Exposure to 100 IU/L FSH significantly influenced the follicle morphology and slowed down nuclear maturation and mucification in in vitro mouse follicles, suggesting that the presence of luteinizing hormone activity tempered the effect of the elevated FSH dose.
Journal ArticleDOI
Lgr4 gene regulates corpus luteum maturation through modulation of the WNT-mediated EGFR-ERK signaling pathway
Hongjie Pan,Hengxiang Cui,Shijie Liu,Yu Qian,Haili Wu,Liang Li,Yuting Guan,Xin Guan,Long Zhang,Heng-Yu Fan,Yanlin Ma,Runsheng Li,Mingyao Liu,Mingyao Liu,Mingyao Liu,Dali Li +15 more
TL;DR: It is reported that Lgr4 modulates WNT-mediated EGFR-ERK signaling to facilitate corpus luteum maturation and ovarian steroidogenesis to maintain female reproduction.
Journal ArticleDOI
Placental overgrowth and fertility defects in mice with a hypermorphic allele of epidermal growth factor receptor
TL;DR: It is demonstrated that mice with increased levels of EGFR signaling exhibit an extensive level of genetic background-dependent phenotypic variability and EGFR expressed in the uterine stroma may play an underappreciated role in preparation of the uterus for embryo implantation.
References
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Journal ArticleDOI
Untangling the ErbB signalling network
Yosef Yarden,Mark X. Sliwkowski +1 more
TL;DR: When epidermal growth factor and its relatives bind the ErbB family of receptors, they trigger a rich network of signalling pathways, culminating in responses ranging from cell division to death, motility to adhesion.
PatentDOI
EGF receptor transactivation by G-protein-coupled receptors requires metalloproteinase cleavage of proHB-EGF
TL;DR: In this article, agents and methods for growth factor receptor activation by modulating the G-protein mediated signal transduction pathway were described, and a method to activate the growth factor receptors was proposed.
Journal ArticleDOI
Epidermal growth factor receptors: critical mediators of multiple receptor pathways.
TL;DR: The receptor for epidermal growth factor was identified as a downstream element in different signaling pathways, expanding its classical function as a receptor for EGF-like ligands to a role as mediator of diverse signaling systems and as a switch point of a cellular communication network.
Journal ArticleDOI
Targeted inactivation of the EGF and amphiregulin genes reveals distinct roles for EGF receptor ligands in mouse mammary gland development
Noreen C. Luetteke,Ting Hu Qiu,Suzanne E. Fenton,Kelly L. Troyer,Richard F. Riedel,Aileen Chang,David C. Lee +6 more
TL;DR: The results provide genetic confirmation of a requirement for EGFR signaling throughout the development of the mouse mammary gland, and reveal stage-dependent activities for different EGFR ligands.