Journal ArticleDOI
EGF-like growth factors as mediators of LH action in the ovulatory follicle.
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TLDR
It is demonstrated that LH stimulation induces the transient and sequential expression of the epidermal growth factor (EGF) family members amphiregulin, epire gulin, and beta-cellulin, which are paracrine mediators that propagate the LH signal throughout the follicle.Abstract:
Before ovulation in mammals, a cascade of events resembling an inflammatory and/or tissue remodeling process is triggered by luteinizing hormone (LH) in the ovarian follicle. Many LH effects, however, are thought to be indirect because of the restricted expression of its receptor. Here, we demonstrate that LH stimulation induces the transient and sequential expression of the epidermal growth factor (EGF) family members amphiregulin, epiregulin, and beta-cellulin. Incubation of follicles with these growth factors recapitulates the morphological and biochemical events triggered by LH, including cumulus expansion and oocyte maturation. Thus, these EGF-related growth factors are paracrine mediators that propagate the LH signal throughout the follicle.read more
Citations
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Journal ArticleDOI
Improved Oocyte Isolation and Embryonic Development of Outbred Deer Mice
Jung Kyu Choi,Xiaoming He +1 more
TL;DR: This study improved the protocol for isolating cumulus-oocyte complexes (COCs) from the outbred deer mice by using only one hormone with reduced dose, and identified that significantly more metaphase II (MII) oocytes could be obtained by supplementing epidermal growth factor (EGF) and leukemia inhibition factor (LIF) into the previously established medium for in vitro maturation (IVM) of the COCs.
Journal ArticleDOI
GnRH actions on rat preovulatory follicles are mediated by paracrine EGF-like factors.
TL;DR: It is demonstrated that follicular EGF‐like factors mediate also the GnRH‐stimulation of ovulatory changes, like these of LH/hCG, and blocked Gn RH‐induced oocyte maturation and follicular progesterone synthesis.
Journal ArticleDOI
The Cebpd (C/EBPδ) Gene Is Induced by Luteinizing Hormones in Ovarian Theca and Interstitial Cells But Is Not Essential for Mouse Ovary Function
A-Mei Huang,Martina Rudelius,Shikha Sharan,Jan M. McAllister,Mark Raffeld,Lane K. Christenson,Esta Sterneck +6 more
TL;DR: It is reported that Cebpd (C/EBPδ) is expressed in the mouse ovary in a highly restricted temporal and spatial pattern and identifies a novel functional unit of mouse theca cells of primary through tertiary follicles responding to LH/hCG together with a subset of interstitial cells.
Journal ArticleDOI
Ovary-specific depletion of the nuclear receptor Nr5a2 compromises expansion of the cumulus oophorus but not fertilization by intracytoplasmic sperm injection.
Kalyne Bertolin,Marie-Charlotte Meinsohn,Joao Suzuki,Jan A. Gossen,Kristina Schoonjans,Rajesha Duggavathi,Bruce D. Murphy +6 more
TL;DR: It is concluded that Nr5a2 is essential for cumulus expansion in granulosa cells throughout follicular development and disrupted in follicular somatic cells does not affect the capacity of the oocyte to be fertilized by intracytoplasmic sperm injection.
Journal ArticleDOI
Proteomics Recapitulates Ovarian Proteins Relevant to Puberty and Fertility in Brahman Heifers (Bos indicus L.).
Muhammad S. Tahir,Loan T. Nguyen,Benjamin L. Schulz,Gry A. Boe-Hansen,Milton G Thomas,Stephen S. Moore,Li Yieng Lau,Marina R. S. Fortes +7 more
TL;DR: The data indicated an up-regulation of steroidogenic proteins contributing to progesterone synthesis at luteal phase post-puberty, and the DA proteins and their pathways were related to ovarian activity in Bos indicus cattle.
References
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Journal ArticleDOI
Untangling the ErbB signalling network
Yosef Yarden,Mark X. Sliwkowski +1 more
TL;DR: When epidermal growth factor and its relatives bind the ErbB family of receptors, they trigger a rich network of signalling pathways, culminating in responses ranging from cell division to death, motility to adhesion.
PatentDOI
EGF receptor transactivation by G-protein-coupled receptors requires metalloproteinase cleavage of proHB-EGF
TL;DR: In this article, agents and methods for growth factor receptor activation by modulating the G-protein mediated signal transduction pathway were described, and a method to activate the growth factor receptors was proposed.
Journal ArticleDOI
Epidermal growth factor receptors: critical mediators of multiple receptor pathways.
TL;DR: The receptor for epidermal growth factor was identified as a downstream element in different signaling pathways, expanding its classical function as a receptor for EGF-like ligands to a role as mediator of diverse signaling systems and as a switch point of a cellular communication network.
Journal ArticleDOI
Targeted inactivation of the EGF and amphiregulin genes reveals distinct roles for EGF receptor ligands in mouse mammary gland development
Noreen C. Luetteke,Ting Hu Qiu,Suzanne E. Fenton,Kelly L. Troyer,Richard F. Riedel,Aileen Chang,David C. Lee +6 more
TL;DR: The results provide genetic confirmation of a requirement for EGFR signaling throughout the development of the mouse mammary gland, and reveal stage-dependent activities for different EGFR ligands.