Journal ArticleDOI
EGF-like growth factors as mediators of LH action in the ovulatory follicle.
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TLDR
It is demonstrated that LH stimulation induces the transient and sequential expression of the epidermal growth factor (EGF) family members amphiregulin, epire gulin, and beta-cellulin, which are paracrine mediators that propagate the LH signal throughout the follicle.Abstract:
Before ovulation in mammals, a cascade of events resembling an inflammatory and/or tissue remodeling process is triggered by luteinizing hormone (LH) in the ovarian follicle. Many LH effects, however, are thought to be indirect because of the restricted expression of its receptor. Here, we demonstrate that LH stimulation induces the transient and sequential expression of the epidermal growth factor (EGF) family members amphiregulin, epiregulin, and beta-cellulin. Incubation of follicles with these growth factors recapitulates the morphological and biochemical events triggered by LH, including cumulus expansion and oocyte maturation. Thus, these EGF-related growth factors are paracrine mediators that propagate the LH signal throughout the follicle.read more
Citations
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Journal ArticleDOI
Anti-bacterial factors secreted from cumulus cells of ovulated COCs enhance sperm capacitation during in vitro fertilization.
TL;DR: The aim of this study was to find immune‐related genes expressed in cumulus cells of ovulated cumulus oocyte complexes and to clear the functional roles during fertilization process.
Journal ArticleDOI
Mitochondrial function in immature bovine oocytes is improved by an increase of cellular cyclic AMP.
Shu Hashimoto,Masaya Yamanaka,Takayuki Yamochi,Hisataka Iwata,Ryouka Kawahara-Miki,Masayasu Inoue,Yoshiharu Morimoto +6 more
TL;DR: It is revealed that forskolin and 3-isobutyl-1-methylxanthine treatment of prophase-stage oocytes induced the expression of genes required for glycolysis, fatty acid degradation, and the mitochondrial electron transport system and improved mitochondrial functions and ATP levels in oocytes without involving nuclear maturation.
Journal ArticleDOI
Improvements in oocyte competence in superovulated mice following treatment with cilostazol: Ovulation of immature oocytes with high developmental rates
Ahmed M. Taiyeb,Saeeda A. Muhsen-Alanssari,William L. Dees,Jill K. Hiney,M. E. Kjelland,Duane C. Kraemer,Mundhir T. Ridha-Albarzanchi +6 more
TL;DR: The capability of cilostazol, a clinically approved medication, to improve mouse oocyte competence suggests the potential benefit of including this compound in ovarian hyperstimulation programs to improve in vitro fertilization outcomes in infertile women.
Regulation of the recruitment and function of T lymphocytes by endothelial and steroidogenic cells
TL;DR: To understand how the process immune cell recruitment into the CL is regulated, a protocol was developed to isolate endothelial cells from the CL and the number of adhered T lymphocytes was determined by fluorescence.
DissertationDOI
MicroRNA expression and function during porcine oocyte maturation and early embryonic development
TL;DR: This chapter describes the construction of a £50,000 house in the Czech Republic over a period of three years and describes the efforts to renovate the property and the conditions that led to its eventual sale.
References
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Journal ArticleDOI
Untangling the ErbB signalling network
Yosef Yarden,Mark X. Sliwkowski +1 more
TL;DR: When epidermal growth factor and its relatives bind the ErbB family of receptors, they trigger a rich network of signalling pathways, culminating in responses ranging from cell division to death, motility to adhesion.
PatentDOI
EGF receptor transactivation by G-protein-coupled receptors requires metalloproteinase cleavage of proHB-EGF
TL;DR: In this article, agents and methods for growth factor receptor activation by modulating the G-protein mediated signal transduction pathway were described, and a method to activate the growth factor receptors was proposed.
Journal ArticleDOI
Epidermal growth factor receptors: critical mediators of multiple receptor pathways.
TL;DR: The receptor for epidermal growth factor was identified as a downstream element in different signaling pathways, expanding its classical function as a receptor for EGF-like ligands to a role as mediator of diverse signaling systems and as a switch point of a cellular communication network.
Journal ArticleDOI
Targeted inactivation of the EGF and amphiregulin genes reveals distinct roles for EGF receptor ligands in mouse mammary gland development
Noreen C. Luetteke,Ting Hu Qiu,Suzanne E. Fenton,Kelly L. Troyer,Richard F. Riedel,Aileen Chang,David C. Lee +6 more
TL;DR: The results provide genetic confirmation of a requirement for EGFR signaling throughout the development of the mouse mammary gland, and reveal stage-dependent activities for different EGFR ligands.