Journal ArticleDOI
EGF-like growth factors as mediators of LH action in the ovulatory follicle.
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TLDR
It is demonstrated that LH stimulation induces the transient and sequential expression of the epidermal growth factor (EGF) family members amphiregulin, epire gulin, and beta-cellulin, which are paracrine mediators that propagate the LH signal throughout the follicle.Abstract:
Before ovulation in mammals, a cascade of events resembling an inflammatory and/or tissue remodeling process is triggered by luteinizing hormone (LH) in the ovarian follicle. Many LH effects, however, are thought to be indirect because of the restricted expression of its receptor. Here, we demonstrate that LH stimulation induces the transient and sequential expression of the epidermal growth factor (EGF) family members amphiregulin, epiregulin, and beta-cellulin. Incubation of follicles with these growth factors recapitulates the morphological and biochemical events triggered by LH, including cumulus expansion and oocyte maturation. Thus, these EGF-related growth factors are paracrine mediators that propagate the LH signal throughout the follicle.read more
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Journal ArticleDOI
Simulated physiological oocyte maturation (SPOM): a novel in vitro maturation system that substantially improves embryo yield and pregnancy outcomes
F. K. Albuz,M. Sasseville,Michelle Lane,David T. Armstrong,Jeremy G. Thompson,Robert B. Gilchrist +5 more
TL;DR: SPOM is a new approach to IVM, mimicing some characteristics of oocyte maturation in vivo and substantially improving oocyte developmental outcomes, which should have significant implications for infertility management and bring important benefits to patients.
Journal ArticleDOI
Gene expression profiles of cumulus cell oocyte complexes during ovulation reveal cumulus cells express neuronal and immune-related genes: does this expand their role in the ovulation process?
Inmaculada Hernandez-Gonzalez,Ignacio Gonzalez-Robayna,Masayuki Shimada,Chad M. Wayne,Scott A. Ochsner,Lisa D. White,JoAnne S. Richards +6 more
TL;DR: There is a cumulus cell-specific, terminal differentiation process and the vast number of matrix, neuronal, and especially immune cell-related genes identified by the gene- profiling data of COCs constitutes strong and novel evidence that cumulus cells possess a repertoire of immune functions that could be far greater than simply mediating an inflammatory-like response.
Journal ArticleDOI
Estrogen Receptor-β Is Critical to Granulosa Cell Differentiation and the Ovulatory Response to Gonadotropins
TL;DR: It is demonstrated that ERbeta-mediated estradiol actions are vital to FSH-induced granulosa cell differentiation; and in the absence of ERbeta, preovulatory follicles are deficient in the necessary cellular organization and receptor signaling pathways.
Journal ArticleDOI
Recent insights into oocyte-follicle cell interactions provide opportunities for the development of new approaches to in vitro maturation.
TL;DR: The challenge for oocyte biologists and clinicians practicing IVM is to modernise clinical IVM systems to benefit from new insights into oocyte-follicle cell interactions in vivo.
Journal ArticleDOI
TSG-6: a pluripotent inflammatory mediator?
TL;DR: The interactions of TSG-6 with HA, heparin and at least some of its protein ligands are sensitive to pH, therefore it seems that in different tissue micro-environments (characterized, for example, by pH and GAG content), TSG -6 could be partitioned into functional pools with distinct activities.
References
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Journal ArticleDOI
Untangling the ErbB signalling network
Yosef Yarden,Mark X. Sliwkowski +1 more
TL;DR: When epidermal growth factor and its relatives bind the ErbB family of receptors, they trigger a rich network of signalling pathways, culminating in responses ranging from cell division to death, motility to adhesion.
PatentDOI
EGF receptor transactivation by G-protein-coupled receptors requires metalloproteinase cleavage of proHB-EGF
TL;DR: In this article, agents and methods for growth factor receptor activation by modulating the G-protein mediated signal transduction pathway were described, and a method to activate the growth factor receptors was proposed.
Journal ArticleDOI
Epidermal growth factor receptors: critical mediators of multiple receptor pathways.
TL;DR: The receptor for epidermal growth factor was identified as a downstream element in different signaling pathways, expanding its classical function as a receptor for EGF-like ligands to a role as mediator of diverse signaling systems and as a switch point of a cellular communication network.
Journal ArticleDOI
Targeted inactivation of the EGF and amphiregulin genes reveals distinct roles for EGF receptor ligands in mouse mammary gland development
Noreen C. Luetteke,Ting Hu Qiu,Suzanne E. Fenton,Kelly L. Troyer,Richard F. Riedel,Aileen Chang,David C. Lee +6 more
TL;DR: The results provide genetic confirmation of a requirement for EGFR signaling throughout the development of the mouse mammary gland, and reveal stage-dependent activities for different EGFR ligands.