Journal ArticleDOI
EGF-like growth factors as mediators of LH action in the ovulatory follicle.
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TLDR
It is demonstrated that LH stimulation induces the transient and sequential expression of the epidermal growth factor (EGF) family members amphiregulin, epire gulin, and beta-cellulin, which are paracrine mediators that propagate the LH signal throughout the follicle.Abstract:
Before ovulation in mammals, a cascade of events resembling an inflammatory and/or tissue remodeling process is triggered by luteinizing hormone (LH) in the ovarian follicle. Many LH effects, however, are thought to be indirect because of the restricted expression of its receptor. Here, we demonstrate that LH stimulation induces the transient and sequential expression of the epidermal growth factor (EGF) family members amphiregulin, epiregulin, and beta-cellulin. Incubation of follicles with these growth factors recapitulates the morphological and biochemical events triggered by LH, including cumulus expansion and oocyte maturation. Thus, these EGF-related growth factors are paracrine mediators that propagate the LH signal throughout the follicle.read more
Citations
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Journal ArticleDOI
Influence of hyaluronic acid synthesis and cumulus mucification on bovine oocyte in vitro maturation, fertilisation and embryo development
Cynthia Gutnisky,Gabriel Carlos Dalvit,Laura N. Pintos,Jeremy G. Thompson,M.T. Beconi,Pablo Daniel Cetica +5 more
TL;DR: Mucification during IVM is independent of cumulus cell proliferation, COC protein content, oocyte meiotic maturation and subsequent developmental competence once fertilised, most likely by facilitating the processes of capacitation and acrosome reaction.
Journal ArticleDOI
Trafficking of epidermal growth factor receptor ligands in polarized epithelial cells.
Bhuminder Singh,Robert J. Coffey +1 more
TL;DR: A rich diversity of apico-basolateral trafficking mechanisms among the EGFR ligands are uncovered, insights are provided into the pathogenesis of an inherited magnesium-wasting disorder of the kidney (isolated renal hypomagnesemia), and a new mode of EGFRligand signaling via exosomes is identified.
Journal ArticleDOI
Growth differentiation factor 9 signaling requires ERK1/2 activity in mouse granulosa and cumulus cells
M. Sasseville,Lesley J. Ritter,Thao Nguyen,Fang Liu,David G. Mottershead,Darryl L. Russell,Robert B. Gilchrist +6 more
TL;DR: Ovarian folliculogenesis is driven by the combined action of endocrine cues and paracrine factors, and oocyte-secreted TGFβ-like ligands and EGFR–ERK1/2 signaling are cooperatively required for the unique granulosa cell response to the signal from oocytes mediating granULosa cell survival and proliferation and hence the promotion of follicle growth and ovulation.
Journal ArticleDOI
Activation of PKA, p38 MAPK and ERK1/2 by gonadotropins in cumulus cells is critical for induction of EGF-like factor and TACE/ADAM17 gene expression during in vitro maturation of porcine COCs
TL;DR: PKA, p38 MAPK and ERK1/2 positively controlled the expression of EGF-like factor and TACE/ADMA17, the latter of which impacts the cumulus expansion and oocyte maturation of porcine COCs via the EGFR-ERK1-2 pathway in cumulus cells.
Journal ArticleDOI
The involvement of the Toll-like receptor family in ovulation
TL;DR: These inflammatory factors exert potent effects on cumulus cell-oocyte expansion, ovulation, transport and fertilization indicating that ovulation is a more complex immune-inflammatory process than previously recognized.
References
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Journal ArticleDOI
Untangling the ErbB signalling network
Yosef Yarden,Mark X. Sliwkowski +1 more
TL;DR: When epidermal growth factor and its relatives bind the ErbB family of receptors, they trigger a rich network of signalling pathways, culminating in responses ranging from cell division to death, motility to adhesion.
PatentDOI
EGF receptor transactivation by G-protein-coupled receptors requires metalloproteinase cleavage of proHB-EGF
TL;DR: In this article, agents and methods for growth factor receptor activation by modulating the G-protein mediated signal transduction pathway were described, and a method to activate the growth factor receptors was proposed.
Journal ArticleDOI
Epidermal growth factor receptors: critical mediators of multiple receptor pathways.
TL;DR: The receptor for epidermal growth factor was identified as a downstream element in different signaling pathways, expanding its classical function as a receptor for EGF-like ligands to a role as mediator of diverse signaling systems and as a switch point of a cellular communication network.
Journal ArticleDOI
Targeted inactivation of the EGF and amphiregulin genes reveals distinct roles for EGF receptor ligands in mouse mammary gland development
Noreen C. Luetteke,Ting Hu Qiu,Suzanne E. Fenton,Kelly L. Troyer,Richard F. Riedel,Aileen Chang,David C. Lee +6 more
TL;DR: The results provide genetic confirmation of a requirement for EGFR signaling throughout the development of the mouse mammary gland, and reveal stage-dependent activities for different EGFR ligands.