The neuropeptide NMU amplifies ILC2-driven allergic lung inflammation
Antonia Wallrapp,Samantha J. Riesenfeld,Patrick R. Burkett,Raja-Elie E. Abdulnour,Jackson Nyman,Danielle Dionne,Matan Hofree,Michael S. Cuoco,Christopher Rodman,Daneyal Farouq,Brian J. Haas,Timothy L. Tickle,John J. Trombetta,Pankaj Baral,Christoph S.N. Klose,Tanel Mahlakõiv,David Artis,Orit Rozenblatt-Rosen,Isaac M. Chiu,Bruce D. Levy,Monika S. Kowalczyk,Aviv Regev,Aviv Regev,Vijay K. Kuchroo,Vijay K. Kuchroo +24 more
TLDR
In this paper, the authors profiled mouse lung-resident ILCs using single-cell RNA sequencing at steady state and after in vivo stimulation with the alarmin cytokines IL-25 and IL-33.Abstract:
Type 2 innate lymphoid cells (ILC2s) both contribute to mucosal homeostasis and initiate pathologic inflammation in allergic asthma. However, the signals that direct ILC2s to promote homeostasis versus inflammation are unclear. To identify such molecular cues, we profiled mouse lung-resident ILCs using single-cell RNA sequencing at steady state and after in vivo stimulation with the alarmin cytokines IL-25 and IL-33. ILC2s were transcriptionally heterogeneous after activation, with subpopulations distinguished by expression of proliferative, homeostatic and effector genes. The neuropeptide receptor Nmur1 was preferentially expressed by ILC2s at steady state and after IL-25 stimulation. Neuromedin U (NMU), the ligand of NMUR1, activated ILC2s in vitro, and in vivo co-administration of NMU with IL-25 strongly amplified allergic inflammation. Loss of NMU-NMUR1 signalling reduced ILC2 frequency and effector function, and altered transcriptional programs following allergen challenge in vivo. Thus, NMUR1 signalling promotes inflammatory ILC2 responses, highlighting the importance of neuro-immune crosstalk in allergic inflammation at mucosal surfaces.read more
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Innate Lymphoid Cells: 10 Years On.
Eric Vivier,David Artis,Marco Colonna,Andreas Diefenbach,James P. Di Santo,James P. Di Santo,Gérard Eberl,Gérard Eberl,Shigeo Koyasu,Richard M. Locksley,Andrew N. J. McKenzie,Reina E. Mebius,Fiona Powrie,Hergen Spits +13 more
TL;DR: The advances in ILC biology over the past decade are distill the advances to refine the nomenclature of ILCs and highlight the importance of I LCs in tissue homeostasis, morphogenesis, metabolism, repair, and regeneration.
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The Cytokines of Asthma
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Systematic comparison of single-cell and single-nucleus RNA-sequencing methods.
Jiarui Ding,Xian Adiconis,Sean Simmons,Monika S. Kowalczyk,Cynthia C. Hession,Nemanja D. Marjanovic,Travis K. Hughes,Marc H. Wadsworth,Tyler Burks,Lan T. Nguyen,John Y. Kwon,Boaz Barak,William Ge,Amanda J. Kedaigle,Shaina Carroll,Shuqiang Li,Nir Hacohen,Nir Hacohen,Orit Rozenblatt-Rosen,Alex K. Shalek,Alexandra-Chloé Villani,Alexandra-Chloé Villani,Aviv Regev,Aviv Regev,Joshua Z. Levin +24 more
TL;DR: To directly compare the methods and avoid processing differences introduced by the existing pipelines, scumi is developed, a flexible computational pipeline that can be used with any single-cell RNA-sequencing method.
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The Human Tumor Atlas Network: Charting Tumor Transitions Across Space and Time at Single-Cell Resolution
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