scispace - formally typeset
Open AccessJournal ArticleDOI

Metadata matters: access to image data in the real world

TLDR
An open standard format for multidimensional microscopy image data is described and it is called on the community to use open image data standards and to insist that all imaging platforms support these file formats.
Abstract
Data sharing is important in the biological sciences to prevent duplication of effort, to promote scientific integrity, and to facilitate and disseminate scientific discovery. Sharing requires centralized repositories, and submission to and utility of these resources require common data formats. This is particularly challenging for multidimensional microscopy image data, which are acquired from a variety of platforms with a myriad of proprietary file formats (PFFs). In this paper, we describe an open standard format that we have developed for microscopy image data. We call on the community to use open image data standards and to insist that all imaging platforms support these file formats. This will build the foundation for an open image data repository.

read more

Citations
More filters
Posted ContentDOI

Dynamic calcium-mediated stress response and recovery signatures in the fungal pathogen, Candida albicans

TL;DR: In this article , the authors used live-cell imaging to observe dynamic Ca2+ spiking as well as slower changes in ambient Ca 2+-GCaMP levels elicited by stress or gene deletion.
Journal ArticleDOI

Histopathological Images Analysis and Predictive Modeling Implemented in Digital Pathology—Current Affairs and Perspectives

TL;DR: In this article , the authors conducted a search in PubMed on the topic of digital pathology and its applications, to quantify the current state of knowledge, and found that computer-aided image analysis has a superior potential to identify, extract and quantify features in more detail compared to the human pathologist's evaluating possibilities.
Journal ArticleDOI

Data-driven microscopy allows for automated context-specific acquisition of high-fidelity image data.

TL;DR: Data-driven microscopy (DDM) as discussed by the authors combines dataindependent and data-dependent steps to synergistically enhance data acquired using different imaging modalities, which can reduce human bias, increase reproducibility, and place single-cell characteristics in the context of the sample population when interpreting microscopy data.
References
More filters
Journal ArticleDOI

Genome-wide atlas of gene expression in the adult mouse brain

Ed S. Lein, +109 more
- 11 Jan 2007 - 
TL;DR: An anatomically comprehensive digital atlas containing the expression patterns of ∼20,000 genes in the adult mouse brain is described, providing an open, primary data resource for a wide variety of further studies concerning brain organization and function.
Journal ArticleDOI

Global Analysis of mRNA Localization Reveals a Prominent Role in Organizing Cellular Architecture and Function

TL;DR: A high-resolution fluorescent in situ hybridization procedure was developed and employed to comprehensively evaluate mRNA localization dynamics during early Drosophila embryogenesis, indicating major roles for mRNA localization in nucleating localized cellular machineries.
Journal ArticleDOI

The Zebrafish Information Network: the zebrafish model organism database

TL;DR: The Zebrafish Information Network (ZFIN) provides an integrated representation of mutants, genes, genetic markers, mapping panels, publications and community resources such as meeting announcements and contact information.
Related Papers (5)

The FAIR Guiding Principles for scientific data management and stewardship