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Open AccessJournal ArticleDOI

Metadata matters: access to image data in the real world

TLDR
An open standard format for multidimensional microscopy image data is described and it is called on the community to use open image data standards and to insist that all imaging platforms support these file formats.
Abstract
Data sharing is important in the biological sciences to prevent duplication of effort, to promote scientific integrity, and to facilitate and disseminate scientific discovery. Sharing requires centralized repositories, and submission to and utility of these resources require common data formats. This is particularly challenging for multidimensional microscopy image data, which are acquired from a variety of platforms with a myriad of proprietary file formats (PFFs). In this paper, we describe an open standard format that we have developed for microscopy image data. We call on the community to use open image data standards and to insist that all imaging platforms support these file formats. This will build the foundation for an open image data repository.

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Citations
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Journal ArticleDOI

Technical Challenges of Enterprise Imaging: HIMSS-SIIM Collaborative White Paper

TL;DR: The technical challenges and solutions for acquiring (capturing) and managing enterprise images, particularly those involving visible light applications, are explored, with particular emphasis on the importance of metadata capture in both order- and encounter-based workflow.
Book ChapterDOI

Segmenting and Tracking Multiple Dividing Targets Using ilastik.

TL;DR: This robust tracking-by-assignment algorithm explicitly makes allowance for false positive detections, undersegmentation, and cell division, and users will be able to track targets of interest in their own data.
Journal ArticleDOI

A New Role for Helicobacter pylori Urease: Contributions to Angiogenesis

TL;DR: The results indicate that besides allowing bacterial colonization of the gastric mucosa, H. pylori’s urease triggers processes that initiate pro-angiogenic responses in different cellular models, which may also play a role in gastric carcinoma development.
Journal ArticleDOI

DestVI identifies continuums of cell types in spatial transcriptomics data

TL;DR: Deconvolution of Spatial Transcriptomics profiles using Variational Inference (DestVI) as mentioned in this paper was proposed to identify continuous variation of the transcriptome within cells of the same type.
Journal ArticleDOI

Analyzing Microbial Population Heterogeneity—Expanding the Toolbox of Microfluidic Single-Cell Cultivations

TL;DR: This review reports recent developments and applications of microfluidic single-cell cultivation technology, highlighting fields of broad interest such as growth, gene expression and antibiotic resistance and susceptibility, and compares prominent software systems that emerged during the last decade, discussing their applicability, opportunities and limitations.
References
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Journal ArticleDOI

Genome-wide atlas of gene expression in the adult mouse brain

Ed S. Lein, +109 more
- 11 Jan 2007 - 
TL;DR: An anatomically comprehensive digital atlas containing the expression patterns of ∼20,000 genes in the adult mouse brain is described, providing an open, primary data resource for a wide variety of further studies concerning brain organization and function.
Journal ArticleDOI

Global Analysis of mRNA Localization Reveals a Prominent Role in Organizing Cellular Architecture and Function

TL;DR: A high-resolution fluorescent in situ hybridization procedure was developed and employed to comprehensively evaluate mRNA localization dynamics during early Drosophila embryogenesis, indicating major roles for mRNA localization in nucleating localized cellular machineries.
Journal ArticleDOI

The Zebrafish Information Network: the zebrafish model organism database

TL;DR: The Zebrafish Information Network (ZFIN) provides an integrated representation of mutants, genes, genetic markers, mapping panels, publications and community resources such as meeting announcements and contact information.
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