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Open AccessJournal ArticleDOI

Metadata matters: access to image data in the real world

TLDR
An open standard format for multidimensional microscopy image data is described and it is called on the community to use open image data standards and to insist that all imaging platforms support these file formats.
Abstract
Data sharing is important in the biological sciences to prevent duplication of effort, to promote scientific integrity, and to facilitate and disseminate scientific discovery. Sharing requires centralized repositories, and submission to and utility of these resources require common data formats. This is particularly challenging for multidimensional microscopy image data, which are acquired from a variety of platforms with a myriad of proprietary file formats (PFFs). In this paper, we describe an open standard format that we have developed for microscopy image data. We call on the community to use open image data standards and to insist that all imaging platforms support these file formats. This will build the foundation for an open image data repository.

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Citations
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Journal ArticleDOI

What Is the Best Candidate to Replace the Tarsus? A Biomechanical, Histological, and Optical Study Comparing Five Grafts

TL;DR: In this article , biomechanical, histological, and optical properties of five commonly used posterior lamella grafts (tarsus, conchal cartilage, sclera, hard palate, and dermis) were assessed by tractometry.
Posted ContentDOI

Separase Protease Activity is Required for Cytokinesis in addition to Chromosome Segregation

TL;DR: It is suggested that the protease activity of separase is required for the exocytosis of RAB-11 vesicles during cortical granule exocyTosis and mitotic cytokinesis and that separase has multiple independent substrates that it must cleave to execute various functions.
Journal ArticleDOI

Antibiotic Drug screening and Image Characterization Toolbox (A.D.I.C.T.): a robust imaging workflow to monitor antibiotic stress response in bacterial cells in vivo

TL;DR: A versatile and robust semi-automated image acquisition, processing and analysis workflow to investigate bacterial cell morphology in a quantitative manner is demonstrated and aims at supporting life-scientists to establish their own imaging-pipeline in order to exploit their data as versatile as possible and in a reproducible manner.
Journal ArticleDOI

Imaging Mitochondrial Dynamics in the Xenopus Central Nervous System (CNS).

TL;DR: In this article, an in vivo time-lapse imaging approach was described to monitor and measure the movement and position of the mitochondria in cells of the developing brain in albino Xenopus laevis tadpoles.
Posted ContentDOI

Human pluripotent stem cell-derived photoreceptors switch from cell autonomous axon extension to non-cell autonomous process pulling during synaptic marker redistribution.

TL;DR: In this paper, the authors used a human pluripotent stem cell (hPSC) reporter line that labels photoreceptors (PRs) and generated retinal organoids in order to study mechanisms of PR process extension.
References
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Journal ArticleDOI

Genome-wide atlas of gene expression in the adult mouse brain

Ed S. Lein, +109 more
- 11 Jan 2007 - 
TL;DR: An anatomically comprehensive digital atlas containing the expression patterns of ∼20,000 genes in the adult mouse brain is described, providing an open, primary data resource for a wide variety of further studies concerning brain organization and function.
Journal ArticleDOI

Global Analysis of mRNA Localization Reveals a Prominent Role in Organizing Cellular Architecture and Function

TL;DR: A high-resolution fluorescent in situ hybridization procedure was developed and employed to comprehensively evaluate mRNA localization dynamics during early Drosophila embryogenesis, indicating major roles for mRNA localization in nucleating localized cellular machineries.
Journal ArticleDOI

The Zebrafish Information Network: the zebrafish model organism database

TL;DR: The Zebrafish Information Network (ZFIN) provides an integrated representation of mutants, genes, genetic markers, mapping panels, publications and community resources such as meeting announcements and contact information.
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