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Open AccessJournal ArticleDOI

Metadata matters: access to image data in the real world

TLDR
An open standard format for multidimensional microscopy image data is described and it is called on the community to use open image data standards and to insist that all imaging platforms support these file formats.
Abstract
Data sharing is important in the biological sciences to prevent duplication of effort, to promote scientific integrity, and to facilitate and disseminate scientific discovery. Sharing requires centralized repositories, and submission to and utility of these resources require common data formats. This is particularly challenging for multidimensional microscopy image data, which are acquired from a variety of platforms with a myriad of proprietary file formats (PFFs). In this paper, we describe an open standard format that we have developed for microscopy image data. We call on the community to use open image data standards and to insist that all imaging platforms support these file formats. This will build the foundation for an open image data repository.

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Citations
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Quantitative SIMS Imaging of Agar-Based Microbial Communities

TL;DR: A method for quantitative SIMS imaging of small molecules in agar-based microbial communities and shows that the relative surface densities of the target biomolecules are substantially different from values inferred through direct intensity comparison and that the developed methodologies can be used to quantitatively compare as many ions as there are available standards.
Journal ArticleDOI

CalTrack: High-Throughput Automated Calcium Transient Analysis in Cardiomyocytes.

TL;DR: CalTrack as mentioned in this paper is a MatLab-based algorithm to monitor fluorescent calcium transients in living cardiomyocytes, including isolated single cells or those contained in 3-dimensional tissues or organoids and to analyze data acquired using photomultiplier tubes or employing line scans.
Journal ArticleDOI

Full volume super-resolution imaging of thick mitotic spindle using 3D AO STED microscope

TL;DR: An automated adaptive optics solution is applied to correct the performance of both STED masks, enhancing robustness and expanding the capabilities of this nanoscopic technique.
Journal ArticleDOI

Protein-Mediated Colloidal Assembly

TL;DR: This work uses protein-protein interactions to drive controlled aggregation of polystyrene microparticles, either through reversible coiled-coil interactions or through intermolecular isopeptide linkages, illustrating a versatile strategy for engineering colloidal systems for use in materials science and biotechnology.
References
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Journal ArticleDOI

Genome-wide atlas of gene expression in the adult mouse brain

Ed S. Lein, +109 more
- 11 Jan 2007 - 
TL;DR: An anatomically comprehensive digital atlas containing the expression patterns of ∼20,000 genes in the adult mouse brain is described, providing an open, primary data resource for a wide variety of further studies concerning brain organization and function.
Journal ArticleDOI

Global Analysis of mRNA Localization Reveals a Prominent Role in Organizing Cellular Architecture and Function

TL;DR: A high-resolution fluorescent in situ hybridization procedure was developed and employed to comprehensively evaluate mRNA localization dynamics during early Drosophila embryogenesis, indicating major roles for mRNA localization in nucleating localized cellular machineries.
Journal ArticleDOI

The Zebrafish Information Network: the zebrafish model organism database

TL;DR: The Zebrafish Information Network (ZFIN) provides an integrated representation of mutants, genes, genetic markers, mapping panels, publications and community resources such as meeting announcements and contact information.
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