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Open AccessJournal ArticleDOI

Metadata matters: access to image data in the real world

TLDR
An open standard format for multidimensional microscopy image data is described and it is called on the community to use open image data standards and to insist that all imaging platforms support these file formats.
Abstract
Data sharing is important in the biological sciences to prevent duplication of effort, to promote scientific integrity, and to facilitate and disseminate scientific discovery. Sharing requires centralized repositories, and submission to and utility of these resources require common data formats. This is particularly challenging for multidimensional microscopy image data, which are acquired from a variety of platforms with a myriad of proprietary file formats (PFFs). In this paper, we describe an open standard format that we have developed for microscopy image data. We call on the community to use open image data standards and to insist that all imaging platforms support these file formats. This will build the foundation for an open image data repository.

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Citations
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Differential roles of the ubiquitin proteasome system and autophagy in the clearance of soluble and aggregated TDP-43 species

TL;DR: It is found that soluble TDP-43 is degraded primarily by the UPS, whereas the clearance of aggregated T DP-43 requires autophagy, andTherapies for clearing excess TTP-43 should target a combination of these pathways.
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SABER amplifies FISH: enhanced multiplexed imaging of RNA and DNA in cells and tissues.

TL;DR: This work introduces signal amplification by exchange reaction (SABER), which endows oligonucleotide-based FISH probes with long, single-stranded DNA concatemers that aggregate a multitude of short complementary fluorescent imager strands, resulting in up to 450-fold signal amplification.
Journal ArticleDOI

Segmentation and quantification of subcellular structures in fluorescence microscopy images using Squassh.

TL;DR: A versatile protocol for a method named 'Squassh' (segmentation and quantification of subcellular shapes), which is used for detecting, delineating and quantifyingSubcellular structures in fluorescence microscopy images, implemented in freely available, user-friendly software.
Journal ArticleDOI

High-Content Screening for Quantitative Cell Biology.

TL;DR: Some recent applications of HCS are described, ranging from the identification of genes required for specific biological processes to the characterization of genetic interactions, and major challenges associated with each are described.
Journal ArticleDOI

Image Data Resource: a bioimage data integration and publication platform

TL;DR: IDR provides an online resource and a software infrastructure that promotes and extends publication and reanalysis of scientific image data and is also an open-source platform for publishing imaging data.
References
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Journal ArticleDOI

Genome-wide atlas of gene expression in the adult mouse brain

Ed S. Lein, +109 more
- 11 Jan 2007 - 
TL;DR: An anatomically comprehensive digital atlas containing the expression patterns of ∼20,000 genes in the adult mouse brain is described, providing an open, primary data resource for a wide variety of further studies concerning brain organization and function.
Journal ArticleDOI

Global Analysis of mRNA Localization Reveals a Prominent Role in Organizing Cellular Architecture and Function

TL;DR: A high-resolution fluorescent in situ hybridization procedure was developed and employed to comprehensively evaluate mRNA localization dynamics during early Drosophila embryogenesis, indicating major roles for mRNA localization in nucleating localized cellular machineries.
Journal ArticleDOI

The Zebrafish Information Network: the zebrafish model organism database

TL;DR: The Zebrafish Information Network (ZFIN) provides an integrated representation of mutants, genes, genetic markers, mapping panels, publications and community resources such as meeting announcements and contact information.
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