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Open AccessJournal ArticleDOI

Metadata matters: access to image data in the real world

TLDR
An open standard format for multidimensional microscopy image data is described and it is called on the community to use open image data standards and to insist that all imaging platforms support these file formats.
Abstract
Data sharing is important in the biological sciences to prevent duplication of effort, to promote scientific integrity, and to facilitate and disseminate scientific discovery. Sharing requires centralized repositories, and submission to and utility of these resources require common data formats. This is particularly challenging for multidimensional microscopy image data, which are acquired from a variety of platforms with a myriad of proprietary file formats (PFFs). In this paper, we describe an open standard format that we have developed for microscopy image data. We call on the community to use open image data standards and to insist that all imaging platforms support these file formats. This will build the foundation for an open image data repository.

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Citations
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Journal ArticleDOI

TFMLAB: A MATLAB toolbox for 4D traction force microscopy

TL;DR: TFMLAB as mentioned in this paper integrates all the computational steps to compute active cellular forces from confocal microscopy images, including image processing, cell segmentation, image alignment, matrix displacement measurement and force recovery.
Posted ContentDOI

The duration of mitosis and daughter cell size are modulated by nutrients in budding yeast

TL;DR: It is shown that most growth of a new daughter cell occurs in mitosis, which suggests that mechanisms that control the extent of growth in Mitosis play a major role in cell size control in budding yeast.
Journal ArticleDOI

Real-time polarization microscopy of fibrillar collagen in histopathology

TL;DR: Polychromatic polarization microscope is evaluated to visualize collagen fibers with an optically generated color representation of fiber orientation and alignment when inspecting the sample by a regular microscope with minor modifications, providing clinically useful insight into stromal composition.
Posted ContentDOI

SABER enables highly multiplexed and amplified detection of DNA and RNA in cells and tissues

TL;DR: SABER is introduced, which endows oligo-based FISH probes with long, single-stranded DNA concatemers that serve as targets for sensitive fluores-cent detection and effectively ampli-fies the signal of probes targeting nucleic acids in fixed cells and tissues.
Journal ArticleDOI

MAARS: a novel high-content acquisition software for the analysis of mitotic defects in fission yeast.

TL;DR: Novel open-source software called the Mitotic Analyzing and Recording System (MAARS) provides automatic single-cell analysis of mitotic defects such as spindle mispositioning or chromosome missegregation.
References
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Journal ArticleDOI

Genome-wide atlas of gene expression in the adult mouse brain

Ed S. Lein, +109 more
- 11 Jan 2007 - 
TL;DR: An anatomically comprehensive digital atlas containing the expression patterns of ∼20,000 genes in the adult mouse brain is described, providing an open, primary data resource for a wide variety of further studies concerning brain organization and function.
Journal ArticleDOI

Global Analysis of mRNA Localization Reveals a Prominent Role in Organizing Cellular Architecture and Function

TL;DR: A high-resolution fluorescent in situ hybridization procedure was developed and employed to comprehensively evaluate mRNA localization dynamics during early Drosophila embryogenesis, indicating major roles for mRNA localization in nucleating localized cellular machineries.
Journal ArticleDOI

The Zebrafish Information Network: the zebrafish model organism database

TL;DR: The Zebrafish Information Network (ZFIN) provides an integrated representation of mutants, genes, genetic markers, mapping panels, publications and community resources such as meeting announcements and contact information.
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