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Open AccessJournal ArticleDOI

Metadata matters: access to image data in the real world

TLDR
An open standard format for multidimensional microscopy image data is described and it is called on the community to use open image data standards and to insist that all imaging platforms support these file formats.
Abstract
Data sharing is important in the biological sciences to prevent duplication of effort, to promote scientific integrity, and to facilitate and disseminate scientific discovery. Sharing requires centralized repositories, and submission to and utility of these resources require common data formats. This is particularly challenging for multidimensional microscopy image data, which are acquired from a variety of platforms with a myriad of proprietary file formats (PFFs). In this paper, we describe an open standard format that we have developed for microscopy image data. We call on the community to use open image data standards and to insist that all imaging platforms support these file formats. This will build the foundation for an open image data repository.

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Citations
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Journal ArticleDOI

Particle fraction is a bioactive cue in granular scaffolds.

TL;DR: A method of freeze-drying microgels that leads to consistent and user-defined particle fractions by weighing the dried microgel powder and reconstituting at known volumes is described, allowing reproducibly study the effect of particle fraction on cell responses, mechanical properties, and mass transport in granular hydrogels.
Journal ArticleDOI

Nuclear SUN1 stabilizes endothelial cell junctions via microtubules to regulate blood vessel formation

- 29 Mar 2023 - 
TL;DR: In this paper , the nuclear-localized linker of the nucleoskeleton and cytoskeleton (LINC) complex protein SUN1 regulates vascular sprouting and endothelial cell-cell junction morphology and function.
Journal ArticleDOI

Setting Up and Running an Advanced Light Microscopy and Imaging Facility

TL;DR: In this unit, a number of issues and considerations from the authors' own experience that they hope will be helpful when planning or setting up a new facility are gathered and presented.
Posted ContentDOI

Labkit: Labeling and Segmentation Toolkit for Big Image Data

TL;DR: LO_SCPLOWABKITC_SCplow as mentioned in this paper is a plugin for microscopy image segmentation that can be applied to single and multi-channel images as well as to timelapse movies.

Foxo transcription factors: methods and protocols

TL;DR: Foxo transcription factors: methods and protocols, Foxo transcription factor: methods, protocols, and methods for deciphering EMTs in animals and plants using a probabilistic approach.
References
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Journal ArticleDOI

Genome-wide atlas of gene expression in the adult mouse brain

Ed S. Lein, +109 more
- 11 Jan 2007 - 
TL;DR: An anatomically comprehensive digital atlas containing the expression patterns of ∼20,000 genes in the adult mouse brain is described, providing an open, primary data resource for a wide variety of further studies concerning brain organization and function.
Journal ArticleDOI

Global Analysis of mRNA Localization Reveals a Prominent Role in Organizing Cellular Architecture and Function

TL;DR: A high-resolution fluorescent in situ hybridization procedure was developed and employed to comprehensively evaluate mRNA localization dynamics during early Drosophila embryogenesis, indicating major roles for mRNA localization in nucleating localized cellular machineries.
Journal ArticleDOI

The Zebrafish Information Network: the zebrafish model organism database

TL;DR: The Zebrafish Information Network (ZFIN) provides an integrated representation of mutants, genes, genetic markers, mapping panels, publications and community resources such as meeting announcements and contact information.
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