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Open AccessJournal ArticleDOI

Metadata matters: access to image data in the real world

TLDR
An open standard format for multidimensional microscopy image data is described and it is called on the community to use open image data standards and to insist that all imaging platforms support these file formats.
Abstract
Data sharing is important in the biological sciences to prevent duplication of effort, to promote scientific integrity, and to facilitate and disseminate scientific discovery. Sharing requires centralized repositories, and submission to and utility of these resources require common data formats. This is particularly challenging for multidimensional microscopy image data, which are acquired from a variety of platforms with a myriad of proprietary file formats (PFFs). In this paper, we describe an open standard format that we have developed for microscopy image data. We call on the community to use open image data standards and to insist that all imaging platforms support these file formats. This will build the foundation for an open image data repository.

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Citations
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Journal ArticleDOI

Transcription factor localization dynamics and DNA binding drive distinct promoter interpretations

- 01 May 2023 - 
TL;DR: In this article , the authors used light-controlled mutants of the yeast TF Msn2 as a model system to investigate how promoter decoding of TF localization dynamics is affected by changes in the ability of the TF to bind DNA.
Journal ArticleDOI

Microglial phagocytosis mediates long-term restructuring of spinal GABAergic circuits following early life injury

TL;DR: In this article , the authors used hindpaw surgical incision as a model to investigate the role of microglia in the response to peripheral injury and found that microglial density and phagocytosis increased in the dorsal horn region.
Posted ContentDOI

Seeing in the dark: Vectashield quenches Alexa Fluor 647 fluorescence, but does not hinder dSTORM imaging

TL;DR: The results suggest that AF647 in Vectashield is not compatible with conventional fluorescence microscopy, because the dye gets quenched, and these findings have important consequences not only for dSTORM, but for any type of fluorescence microscopeopy.
References
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Journal ArticleDOI

Genome-wide atlas of gene expression in the adult mouse brain

Ed S. Lein, +109 more
- 11 Jan 2007 - 
TL;DR: An anatomically comprehensive digital atlas containing the expression patterns of ∼20,000 genes in the adult mouse brain is described, providing an open, primary data resource for a wide variety of further studies concerning brain organization and function.
Journal ArticleDOI

Global Analysis of mRNA Localization Reveals a Prominent Role in Organizing Cellular Architecture and Function

TL;DR: A high-resolution fluorescent in situ hybridization procedure was developed and employed to comprehensively evaluate mRNA localization dynamics during early Drosophila embryogenesis, indicating major roles for mRNA localization in nucleating localized cellular machineries.
Journal ArticleDOI

The Zebrafish Information Network: the zebrafish model organism database

TL;DR: The Zebrafish Information Network (ZFIN) provides an integrated representation of mutants, genes, genetic markers, mapping panels, publications and community resources such as meeting announcements and contact information.
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