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Open AccessJournal ArticleDOI

Metadata matters: access to image data in the real world

TLDR
An open standard format for multidimensional microscopy image data is described and it is called on the community to use open image data standards and to insist that all imaging platforms support these file formats.
Abstract
Data sharing is important in the biological sciences to prevent duplication of effort, to promote scientific integrity, and to facilitate and disseminate scientific discovery. Sharing requires centralized repositories, and submission to and utility of these resources require common data formats. This is particularly challenging for multidimensional microscopy image data, which are acquired from a variety of platforms with a myriad of proprietary file formats (PFFs). In this paper, we describe an open standard format that we have developed for microscopy image data. We call on the community to use open image data standards and to insist that all imaging platforms support these file formats. This will build the foundation for an open image data repository.

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Patent

Signatures and determinants associated with prostate cancer progression and methods of use thereof

TL;DR: In this paper, a set of DETERMINANTS (e.g., genes and gene products) are used to accurately inform about the risk of cancer progression and recurrence, as well as methods of their use.
Journal ArticleDOI

Light-dependent single-cell heterogeneity in the chloroplast redox state regulates cell fate in a marine diatom.

TL;DR: It is proposed that light-dependent metabolic heterogeneity regulates diatoms’ sensitivity to environmental stressors in the ocean and is used for sensing stress cues and regulating cell fate.
Journal ArticleDOI

Dendritic Cell Vaccination in Metastatic Melanoma Turns "Non-T Cell Inflamed" Into "T-Cell Inflamed" Tumors.

TL;DR: Light is shed on DC immunotherapy-induced TME changes, lending the rationale for the design of smarter immune-combination therapies and indicating that DC vaccination exerts an important role in sustaining or de novo inducing a T cell inflamed TME.
Journal ArticleDOI

Workflows for microscopy image analysis and cellular phenotyping

TL;DR: In this review, workflow systems for the integration of microscopy image analysis techniques with focus on KNIME and Galaxy are described.
References
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Journal ArticleDOI

Genome-wide atlas of gene expression in the adult mouse brain

Ed S. Lein, +109 more
- 11 Jan 2007 - 
TL;DR: An anatomically comprehensive digital atlas containing the expression patterns of ∼20,000 genes in the adult mouse brain is described, providing an open, primary data resource for a wide variety of further studies concerning brain organization and function.
Journal ArticleDOI

Global Analysis of mRNA Localization Reveals a Prominent Role in Organizing Cellular Architecture and Function

TL;DR: A high-resolution fluorescent in situ hybridization procedure was developed and employed to comprehensively evaluate mRNA localization dynamics during early Drosophila embryogenesis, indicating major roles for mRNA localization in nucleating localized cellular machineries.
Journal ArticleDOI

The Zebrafish Information Network: the zebrafish model organism database

TL;DR: The Zebrafish Information Network (ZFIN) provides an integrated representation of mutants, genes, genetic markers, mapping panels, publications and community resources such as meeting announcements and contact information.
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The FAIR Guiding Principles for scientific data management and stewardship