Exosome Analysis in Tumor-Draining Pulmonary Vein Identifies NSCLC Patients with Higher Risk of Relapse after Curative Surgery
Alfons Navarro,Laureano Molins,Ramon M. Marrades,Jorge Moisés,Nuria Viñolas,Sara Morales,Jordi Canals,Joan J. Castellano,José Ramírez,Mariano Monzo +9 more
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TLDR
PV exosome size is a promising relapse biomarker after surgery that can add valuable information to clinical variables and be associated with shorter TTR and OS.Abstract:
Since tumor-draining pulmonary vein blood (PV) is enriched in tumor-secreted products, we hypothesized that it would also be enriched in tumor-derived exosomes, which would be important in the metastasis process. We characterized exosomes from PV of 61 resected non-small cell lung cancer (NSCLC) patients to evaluate its potential as relapse biomarkers. Exosomes were characterized using transmission electron microscopy, western blot and nanoparticle tracking analysis and we examined time to relapse (TTR) and overall survival (OS). Differences between PV and peripheral vein were found. PV was enriched in smaller exosomes than the paired peripheral vein (p = 0.01). Moreover, PV exosome size mode was able to identify relapsed patients (Area under the curve [AUC] = 0.781; 95%CI: 0.6641⁻0.8978), in whom exosome size was smaller (<112 nm; p < 0.001). The combination of PV exosome size and N (lymph node involvement) showed an AUC of 0.89 (95%CI: 0.80⁻0.97). Moreover, smaller PV exosome size was associated with shorter TTR (28.3 months vs. not reached, p < 0.001) and OS (43.9 months vs. not reached, p = 0.009). Multivariate analyses identified PV exosome size and stage as independent prognostic markers for TTR and OS. PV exosome size is a promising relapse biomarker after surgery that can add valuable information to clinical variables.read more
Citations
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Exosome-mediated siRNA delivery to suppress postoperative breast cancer metastasis
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Guangpeng He,Xueqiang Peng,Shibo Wei,Shuo Yang,Xinyu Li,Ming-Lih Huang,Shilei Tang,Hongyuan Jin,Jiaxing Liu,Sheng Zhang,Hongyu Zheng,Qing Fan,Jingang Liu,Liang Wang,Han Li +14 more
TL;DR: In this article , a review of cancer-derived exosomes and their functions under hypoxia is presented, and the size change of hypoxic cancer cell derived exosome is investigated.
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Extracellular Vesicle lincRNA-p21 Expression in Tumor-Draining Pulmonary Vein Defines Prognosis in NSCLC and Modulates Endothelial Cell Behavior
Joan J. Castellano,Ramon M. Marrades,Laureano Molins,Nuria Viñolas,Jorge Moisés,Jordi Canals,Bing Han,Yan Li,Daniel Martinez,Mariano Monzo,Alfons Navarro +10 more
TL;DR: Evaluating the presence of lincRNA-p21 in extracellular vesicles from NSCLC patients and assessing its potential as a prognostic biomarker found it to be a novel prognosis marker, promoting angiogenesis and metastasis.
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