Matrix elasticity directs stem cell lineage specification.
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TLDR
Naive mesenchymal stem cells are shown here to specify lineage and commit to phenotypes with extreme sensitivity to tissue-level elasticity, consistent with the elasticity-insensitive commitment of differentiated cell types.About:
This article is published in Cell.The article was published on 2006-08-25 and is currently open access. It has received 12204 citations till now. The article focuses on the topics: Mesenchymal stem cell differentiation & Stem cell fate determination.read more
Citations
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Patterning hydrogels in three dimensions towards controlling cellular interactions
Sudhir Khetan,Jason A. Burdick +1 more
TL;DR: These techniques can be separated into two categories: stereolithographic “bottom-up” methods that pattern structures during layer-by-layer fabrication and post-gelation techniques involving modification of a uniform, pre-formed hydrogel.
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Advancing Frontiers in Bone Bioprinting.
Nureddin Ashammakhi,Anwarul Hasan,Outi Kaarela,Batzaya Byambaa,Batzaya Byambaa,Amir Sheikhi,Akhilesh K. Gaharwar,Ali Khademhosseini +7 more
TL;DR: A critical overview of the recent literature on various types of bioinks used for bone bioprinting is presented and major challenges, such as the vascularity, clinically relevant size, and mechanical properties of 3D printed structures, are discussed.
Journal ArticleDOI
A mini-review: Cell response to microscale, nanoscale, and hierarchical patterning of surface structure
TL;DR: In this paper, the relationship between cellular responses and physically patterned surfaces is discussed, which could be applied to various biomedical scaffolds used in tissue engineering applications, and fabrication methods for obtaining physically-patterned microscale and nanoscale surfaces are reviewed.
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Biomaterials for tissue engineering applications.
TL;DR: The pediatric community, therefore, provides a distinct challenge for the tissue engineering community and biomaterials are a fundamental component of tissue engineering, which aims to replace diseased, damaged, or missing tissue with reconstructed functional tissue.
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Cell-scaffold mechanical interplay within engineered tissue.
Dekel Dado,Shulamit Levenberg +1 more
TL;DR: This review summarizes the mechanical interplay between cells and scaffold within engineered tissue and addresses some theories related to the effects of scaffold stiffness and mechanical stimulation on cell behavior.
References
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Multilineage Potential of Adult Human Mesenchymal Stem Cells
Mark F. Pittenger,Alastair Morgan Mackay,Stephen C. Beck,Rama K. Jaiswal,Robin Douglas,Joseph D. Mosca,Mark Aaron Moorman,Donald William Jr. Ward Road Simonetti,Stewart Craig,Daniel R. Marshak +9 more
TL;DR: Adult stem cells isolated from marrow aspirates of volunteer donors could be induced to differentiate exclusively into the adipocytic, chondrocytic, or osteocytic lineages.
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Tissue Cells Feel and Respond to the Stiffness of Their Substrate
TL;DR: An understanding of how tissue cells—including fibroblasts, myocytes, neurons, and other cell types—sense matrix stiffness is just emerging with quantitative studies of cells adhering to gels with which elasticity can be tuned to approximate that of tissues.
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CellProfiler: image analysis software for identifying and quantifying cell phenotypes
Anne E. Carpenter,Thouis R. Jones,Michael R. Lamprecht,Colin Clarke,In Han Kang,Ola Friman,David A. Guertin,Joo Han Chang,Robert A. Lindquist,Jason Moffat,Polina Golland,David M. Sabatini +11 more
TL;DR: The first free, open-source system designed for flexible, high-throughput cell image analysis, CellProfiler is described, which can address a variety of biological questions quantitatively.
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Cell shape, cytoskeletal tension, and rhoa regulate stem cell lineage commitment
TL;DR: It is demonstrated that cell shape regulates commitment of human mesenchymal stem cells to adipocyte or osteoblast fate and mechanical cues experienced in developmental and adult contexts, embodied by cell shape, cytoskeletal tension, and RhoA signaling, are integral to the commitment of stem cell fate.
Journal ArticleDOI
Myofibroblasts and mechano-regulation of connective tissue remodelling
TL;DR: It is clear that the understanding of the myofibroblast — its origins, functions and molecular regulation — will have a profound influence on the future effectiveness not only of tissue engineering but also of regenerative medicine generally.
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