Matrix elasticity directs stem cell lineage specification.
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TLDR
Naive mesenchymal stem cells are shown here to specify lineage and commit to phenotypes with extreme sensitivity to tissue-level elasticity, consistent with the elasticity-insensitive commitment of differentiated cell types.About:
This article is published in Cell.The article was published on 2006-08-25 and is currently open access. It has received 12204 citations till now. The article focuses on the topics: Mesenchymal stem cell differentiation & Stem cell fate determination.read more
Citations
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Surface attachment induces Pseudomonas aeruginosa virulence
TL;DR: A rapid imaging-based virulence assay is demonstrated that P. aeruginosa activates virulence in response to attachment to a range of chemically distinct surfaces, suggesting that this bacterial species responds to mechanical properties of its substrates.
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Targeting the cytoskeleton to direct pancreatic differentiation of human pluripotent stem cells
Nathaniel J. Hogrebe,Punn Augsornworawat,Kristina G. Maxwell,Leonardo Velazco-Cruz,Jeffrey R. Millman +4 more
TL;DR: A link between the state of the actin cytoskeleton and the expression of pancreatic transcription factors that drive pancreatic lineage specification is established and Generation of pancreatIC β cells from stem cells is enhanced by manipulating the cytos skeleton.
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Stem Cell Differentiation is Regulated by Extracellular Matrix Mechanics.
TL;DR: Stem cells mechanosense the stiffness of their microenvironment, which impacts differentiation, and extracellular matrix stiffness is clearly transduced into gene expression via adhesion and cytoskeleton proteins that tune fates.
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On scaffold designing for bone regeneration: A computational multiscale approach.
TL;DR: Results show an increasing rate of bone regeneration with increasing scaffold stiffness, scaffold mean pore size and pre-seeding, whereas the collapse of the scaffold occurs for a faster biomaterial resorption kinetics.
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Nuclear deformability and telomere dynamics are regulated by cell geometric constraints.
TL;DR: It is observed that constrained and isotropic cells, which lack long actin stress fibers, have more deformable nuclei than elongated and polarized cells, and active forces from the cytoskeleton and rigidity from lamin A/C nucleoskeleton can together regulate nuclear and chromatin dynamics.
References
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Journal ArticleDOI
Multilineage Potential of Adult Human Mesenchymal Stem Cells
Mark F. Pittenger,Alastair Morgan Mackay,Stephen C. Beck,Rama K. Jaiswal,Robin Douglas,Joseph D. Mosca,Mark Aaron Moorman,Donald William Jr. Ward Road Simonetti,Stewart Craig,Daniel R. Marshak +9 more
TL;DR: Adult stem cells isolated from marrow aspirates of volunteer donors could be induced to differentiate exclusively into the adipocytic, chondrocytic, or osteocytic lineages.
Journal ArticleDOI
Tissue Cells Feel and Respond to the Stiffness of Their Substrate
TL;DR: An understanding of how tissue cells—including fibroblasts, myocytes, neurons, and other cell types—sense matrix stiffness is just emerging with quantitative studies of cells adhering to gels with which elasticity can be tuned to approximate that of tissues.
Journal ArticleDOI
CellProfiler: image analysis software for identifying and quantifying cell phenotypes
Anne E. Carpenter,Thouis R. Jones,Michael R. Lamprecht,Colin Clarke,In Han Kang,Ola Friman,David A. Guertin,Joo Han Chang,Robert A. Lindquist,Jason Moffat,Polina Golland,David M. Sabatini +11 more
TL;DR: The first free, open-source system designed for flexible, high-throughput cell image analysis, CellProfiler is described, which can address a variety of biological questions quantitatively.
Journal ArticleDOI
Cell shape, cytoskeletal tension, and rhoa regulate stem cell lineage commitment
TL;DR: It is demonstrated that cell shape regulates commitment of human mesenchymal stem cells to adipocyte or osteoblast fate and mechanical cues experienced in developmental and adult contexts, embodied by cell shape, cytoskeletal tension, and RhoA signaling, are integral to the commitment of stem cell fate.
Journal ArticleDOI
Myofibroblasts and mechano-regulation of connective tissue remodelling
TL;DR: It is clear that the understanding of the myofibroblast — its origins, functions and molecular regulation — will have a profound influence on the future effectiveness not only of tissue engineering but also of regenerative medicine generally.
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