Matrix elasticity directs stem cell lineage specification.
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TLDR
Naive mesenchymal stem cells are shown here to specify lineage and commit to phenotypes with extreme sensitivity to tissue-level elasticity, consistent with the elasticity-insensitive commitment of differentiated cell types.About:
This article is published in Cell.The article was published on 2006-08-25 and is currently open access. It has received 12204 citations till now. The article focuses on the topics: Mesenchymal stem cell differentiation & Stem cell fate determination.read more
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A critical evaluation of in vitro cell culture models for high-throughput drug screening and toxicity
TL;DR: Common methods of in vitro testing, including dissociated, organotypic, organ/explant, and 3-D cultures, are reviewed here with specific focus on retaining cell and molecular interactions and physiological parameters that determine cell phenotypes and their corresponding responses to bioactive agents.
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High-strength silk protein scaffolds for bone repair
TL;DR: Development of a high compressive strength polymeric bone composite materials is reported, based on silk protein-protein interfacial bonding, which favored human bone marrow-derived mesenchymal stem cell differentiation toward bone-like tissue in vitro based on biochemical and gene expression for bone markers.
Journal ArticleDOI
Noninvasive Assessment of Collagen Gel Microstructure and Mechanics Using Multiphoton Microscopy
Christopher B. Raub,Vinod Suresh,Tatiana B. Krasieva,Julia G. Lyubovitsky,Justin D. Mih,Andrew J. Putnam,Bruce J. Tromberg,Steven C. George +7 more
TL;DR: It is concluded that SHG and TPF can characterize differential microscopic features of the collagen hydrogel that are strongly correlated with bulk mechanical properties and may be a useful noninvasive tool to assess tissue mechanics.
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Nanotopography Modulates Mechanotransduction of Stem Cells and Induces Differentiation through Focal Adhesion Kinase
Benjamin Kim Kiat Teo,Sum Thai Wong,Sum Thai Wong,Choon Kiat Lim,Terrence Y. S. Kung,Chong Hao Yap,Yamini Ramagopal,Lewis H. Romer,Evelyn K.F. Yim +8 more
TL;DR: The results indicated that FAK activity had a direct impact on topography-induced gene expression, and that this effect of FAK was independent of cell shape, suggesting that hMSC sense and transduce nanotopographical signals through focal adhesions and actomyosin cytoskeleton contractility to induce differential gene expression.
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Biomaterials for the Development of Peripheral Nerve Guidance Conduits
TL;DR: Approaches to peripheral nerve repair through a channel or conduit are highlighted, implementing chemical and physical growth and guidance cues to direct that repair process.
References
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Journal ArticleDOI
Multilineage Potential of Adult Human Mesenchymal Stem Cells
Mark F. Pittenger,Alastair Morgan Mackay,Stephen C. Beck,Rama K. Jaiswal,Robin Douglas,Joseph D. Mosca,Mark Aaron Moorman,Donald William Jr. Ward Road Simonetti,Stewart Craig,Daniel R. Marshak +9 more
TL;DR: Adult stem cells isolated from marrow aspirates of volunteer donors could be induced to differentiate exclusively into the adipocytic, chondrocytic, or osteocytic lineages.
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Tissue Cells Feel and Respond to the Stiffness of Their Substrate
TL;DR: An understanding of how tissue cells—including fibroblasts, myocytes, neurons, and other cell types—sense matrix stiffness is just emerging with quantitative studies of cells adhering to gels with which elasticity can be tuned to approximate that of tissues.
Journal ArticleDOI
CellProfiler: image analysis software for identifying and quantifying cell phenotypes
Anne E. Carpenter,Thouis R. Jones,Michael R. Lamprecht,Colin Clarke,In Han Kang,Ola Friman,David A. Guertin,Joo Han Chang,Robert A. Lindquist,Jason Moffat,Polina Golland,David M. Sabatini +11 more
TL;DR: The first free, open-source system designed for flexible, high-throughput cell image analysis, CellProfiler is described, which can address a variety of biological questions quantitatively.
Journal ArticleDOI
Cell shape, cytoskeletal tension, and rhoa regulate stem cell lineage commitment
TL;DR: It is demonstrated that cell shape regulates commitment of human mesenchymal stem cells to adipocyte or osteoblast fate and mechanical cues experienced in developmental and adult contexts, embodied by cell shape, cytoskeletal tension, and RhoA signaling, are integral to the commitment of stem cell fate.
Journal ArticleDOI
Myofibroblasts and mechano-regulation of connective tissue remodelling
TL;DR: It is clear that the understanding of the myofibroblast — its origins, functions and molecular regulation — will have a profound influence on the future effectiveness not only of tissue engineering but also of regenerative medicine generally.
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