Matrix elasticity directs stem cell lineage specification.
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TLDR
Naive mesenchymal stem cells are shown here to specify lineage and commit to phenotypes with extreme sensitivity to tissue-level elasticity, consistent with the elasticity-insensitive commitment of differentiated cell types.About:
This article is published in Cell.The article was published on 2006-08-25 and is currently open access. It has received 12204 citations till now. The article focuses on the topics: Mesenchymal stem cell differentiation & Stem cell fate determination.read more
Citations
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Journal ArticleDOI
Matrix stiffness regulation of integrin‐mediated mechanotransduction during osteogenic differentiation of human mesenchymal stem cells
TL;DR: Evidence is provided that the matrix rigidity affects the osteogenic outcome of MSCs through mechanotransduction events that are mediated by α2‐integrin.
Journal ArticleDOI
Resolution doubling in live, multicellular organisms via multifocal structured illumination microscopy
Andrew York,Sapun H. Parekh,Sapun H. Parekh,Damian Dalle Nogare,Robert S. Fischer,Kelsey Temprine,Marina Mione,Ajay B. Chitnis,Christian A. Combs,Hari Shroff +9 more
TL;DR: Sparse multifocal illumination patterns generated by a digital micromirror device allowed us to physically reject out-of-focus light, enabling 3D subdiffractive imaging in samples eightfold thicker than had been previously imaged with SIM.
Journal ArticleDOI
The effect of matrix stiffness on mesenchymal stem cell differentiation in a 3D thixotropic gel.
TL;DR: The usefulness of thixotropic gels for 3D cell culture studies, as well as the use of tau(y) as an effective measure of matrix stiffness that could be correlated to MSC differentiation are demonstrated.
Book ChapterDOI
Cell Responses to Surface and Architecture of Tissue Engineering Scaffolds
Hsin-I Chang,Yiwei Wang +1 more
TL;DR: The present chapter will discuss cell responses to surface chemistry and various architecture parameters; current approaches and technologies to optimize tissue engineering scaffolds and challenges in studying the cell interaction with scaffolds.
Journal ArticleDOI
Traction forces and rigidity sensing regulate cell functions
Marion Ghibaudo,Alexandre Saez,Léa Trichet,Alain Xayaphoummine,Julien Browaeys,Pascal Silberzan,Axel Buguin,Benoit Ladoux +7 more
TL;DR: An elastic model that estimates the equivalent Young's modulus of a micropillar substrate is proposed, which gives comparable results for both experimental approaches and is proposed to compare the force measurements on micro-textured surfaces and continuous flexible gels.
References
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Journal ArticleDOI
Multilineage Potential of Adult Human Mesenchymal Stem Cells
Mark F. Pittenger,Alastair Morgan Mackay,Stephen C. Beck,Rama K. Jaiswal,Robin Douglas,Joseph D. Mosca,Mark Aaron Moorman,Donald William Jr. Ward Road Simonetti,Stewart Craig,Daniel R. Marshak +9 more
TL;DR: Adult stem cells isolated from marrow aspirates of volunteer donors could be induced to differentiate exclusively into the adipocytic, chondrocytic, or osteocytic lineages.
Journal ArticleDOI
Tissue Cells Feel and Respond to the Stiffness of Their Substrate
TL;DR: An understanding of how tissue cells—including fibroblasts, myocytes, neurons, and other cell types—sense matrix stiffness is just emerging with quantitative studies of cells adhering to gels with which elasticity can be tuned to approximate that of tissues.
Journal ArticleDOI
CellProfiler: image analysis software for identifying and quantifying cell phenotypes
Anne E. Carpenter,Thouis R. Jones,Michael R. Lamprecht,Colin Clarke,In Han Kang,Ola Friman,David A. Guertin,Joo Han Chang,Robert A. Lindquist,Jason Moffat,Polina Golland,David M. Sabatini +11 more
TL;DR: The first free, open-source system designed for flexible, high-throughput cell image analysis, CellProfiler is described, which can address a variety of biological questions quantitatively.
Journal ArticleDOI
Cell shape, cytoskeletal tension, and rhoa regulate stem cell lineage commitment
TL;DR: It is demonstrated that cell shape regulates commitment of human mesenchymal stem cells to adipocyte or osteoblast fate and mechanical cues experienced in developmental and adult contexts, embodied by cell shape, cytoskeletal tension, and RhoA signaling, are integral to the commitment of stem cell fate.
Journal ArticleDOI
Myofibroblasts and mechano-regulation of connective tissue remodelling
TL;DR: It is clear that the understanding of the myofibroblast — its origins, functions and molecular regulation — will have a profound influence on the future effectiveness not only of tissue engineering but also of regenerative medicine generally.
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