Matrix elasticity directs stem cell lineage specification.
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TLDR
Naive mesenchymal stem cells are shown here to specify lineage and commit to phenotypes with extreme sensitivity to tissue-level elasticity, consistent with the elasticity-insensitive commitment of differentiated cell types.About:
This article is published in Cell.The article was published on 2006-08-25 and is currently open access. It has received 12204 citations till now. The article focuses on the topics: Mesenchymal stem cell differentiation & Stem cell fate determination.read more
Citations
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Enhanced Proliferation and Osteogenic Differentiation of Mesenchymal Stem Cells on Graphene Oxide-Incorporated Electrospun Poly(lactic-co-glycolic acid) Nanofibrous Mats
Yu Luo,Yu Luo,He Shen,Yongxiang Fang,Yuhua Cao,Jie Huang,Mengxin Zhang,Jianwu Dai,Xiangyang Shi,Zhijun Zhang +9 more
TL;DR: The fabrication of graphene oxide (GO)-doped poly(lactic-co-glycolic acid) (PLGA) nanofiber scaffolds via electrospinning technique for the enhancement of osteogenic differentiation of MSCs is reported.
Journal ArticleDOI
Cytotoxic T Cells Use Mechanical Force to Potentiate Target Cell Killing.
Roshni Basu,Benjamin M. Whitlock,Julien Husson,Audrey Le Floc’h,Weiyang Jin,Alon Oyler-Yaniv,Farokh Dotiwala,Grégory Giannone,Claire Hivroz,Nicolas Biais,Judy Lieberman,Lance C. Kam,Morgan Huse +12 more
TL;DR: An unappreciated physical dimension to lymphocyte function is revealed and cells use mechanical forces to control the activity of outgoing chemical signals and data indicate that CTLs coordinate perforin release and force exertion in space and time.
Journal ArticleDOI
The mechanical memory of lung myofibroblasts
TL;DR: Mechano-sensed information relating to physical conditions of the local cellular environment could permanently induce fibrotic behavior of lung fibroblasts through priming, which has important implications for the progression and persistence of aggressive Fibrotic diseases such as idiopathic pulmonary fibrosis.
Journal ArticleDOI
Control of cell morphology and differentiation by substrates with independently tunable elasticity and viscous dissipation
TL;DR: A viscoelastic solid hydrogel, based on polyacrylamide, that can be tuned to closely resemble soft tissue, and show the influence of viscous dissipation on cellular mechanical sensing is developed.
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Crawling from soft to stiff matrix polarizes the cytoskeleton and phosphoregulates myosin-II heavy chain
TL;DR: Cytoskeletal polarization occurs in response to mechanosensing of a transition from soft to stiff matrix during migration and promotes dephosphorylation of myOSin-IIA, rearward localization of myosin-IIB, and durotaxis.
References
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Journal ArticleDOI
Multilineage Potential of Adult Human Mesenchymal Stem Cells
Mark F. Pittenger,Alastair Morgan Mackay,Stephen C. Beck,Rama K. Jaiswal,Robin Douglas,Joseph D. Mosca,Mark Aaron Moorman,Donald William Jr. Ward Road Simonetti,Stewart Craig,Daniel R. Marshak +9 more
TL;DR: Adult stem cells isolated from marrow aspirates of volunteer donors could be induced to differentiate exclusively into the adipocytic, chondrocytic, or osteocytic lineages.
Journal ArticleDOI
Tissue Cells Feel and Respond to the Stiffness of Their Substrate
TL;DR: An understanding of how tissue cells—including fibroblasts, myocytes, neurons, and other cell types—sense matrix stiffness is just emerging with quantitative studies of cells adhering to gels with which elasticity can be tuned to approximate that of tissues.
Journal ArticleDOI
CellProfiler: image analysis software for identifying and quantifying cell phenotypes
Anne E. Carpenter,Thouis R. Jones,Michael R. Lamprecht,Colin Clarke,In Han Kang,Ola Friman,David A. Guertin,Joo Han Chang,Robert A. Lindquist,Jason Moffat,Polina Golland,David M. Sabatini +11 more
TL;DR: The first free, open-source system designed for flexible, high-throughput cell image analysis, CellProfiler is described, which can address a variety of biological questions quantitatively.
Journal ArticleDOI
Cell shape, cytoskeletal tension, and rhoa regulate stem cell lineage commitment
TL;DR: It is demonstrated that cell shape regulates commitment of human mesenchymal stem cells to adipocyte or osteoblast fate and mechanical cues experienced in developmental and adult contexts, embodied by cell shape, cytoskeletal tension, and RhoA signaling, are integral to the commitment of stem cell fate.
Journal ArticleDOI
Myofibroblasts and mechano-regulation of connective tissue remodelling
TL;DR: It is clear that the understanding of the myofibroblast — its origins, functions and molecular regulation — will have a profound influence on the future effectiveness not only of tissue engineering but also of regenerative medicine generally.
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